Citrus Tristeza Virus, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2015

Coordinators: Catara Antonino F., Bar-Joseph Moshe, Licciardello Grazia

Language: English

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Citrus Tristeza Virus
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Citrus Tristeza Virus
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247 p. · 17.8x25.4 cm · Hardback
This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.

1. A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies

            Moshe Bar-Joseph

 

2. A Brief Historical Account of the Family Closteroviridae

            Giovanni P. Martelli

 

3. Phenotyping Biological Properties of CTV Isolates

            Marcella Russo and Antonino F. Catara

 

4. CTV Vectors and Interactions with the Virus and Host Plants

            Raymond Yokomi

 

5. Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues

            Mariano Cambra, Eduardo Vidal, Carmen Martínez, and Edson Bertolini

 

6. Detection of Citrus Tristeza Virus and Co-Infecting Viroids

            Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, and Francesco Di Serio

 

7. Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP

            Elisavet K. Chatzivassiliou and Grazia Licciardello

 

8. Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus

            Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, and Raymond Yokomi

 

9. Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device

            Giuseppe Scuderi, Antonino Catara, and Grazia Licciardello

 

10. Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay

            Dilip Kumar Ghosh, Ashish Warghane, and Kajal Kumar Biswas

 

11. Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome

            Munir Mawassi, Sabrina Haviv, and Ludmila Meslenin

 

12. Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus

            Ana Belén Ruiz-García, Rachelle Bester, Antonio Olmos, and Hans Jacob Maree

 

13. Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology

            David A. Read and Gerhard Pietersen

 

14. Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction

            Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio, and Ricardo Flores

 

15. Proteomic Response of Host Plants to Citrus Tristeza Virus

            Milena Santos Dória and Carlos Priminho Pirovani

 

16. Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment

            Yosvanis Acanda, Chunxia Wang, and Amit Levy

 

17. Methods for Producing Transgenic Plants Resistant to CTV

            Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Peña

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts