Crop Breeding, 2014
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1145

The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideo type, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow?s crop varieties.

1. Defining a Genetic Ideotype for Crop Improvement

                Richard M. Trethowan

2. From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding

                Melissa Garcia and Diane E. Mather

3. Temperature Switch PCR (TSP) – A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms

                Thanh Le Phuoc and Kelvin Khoo

4. Multiplex-Ready Technology for Mid-Throughput Genotyping of Molecular Markers

                Julien Bonneau and Matthew Hayden

5. Genotyping by High-Resolution Melting Analysis

                Elise J. Tucker and Bao Lam Huynh

6. Bi-Allelic SNP Genotyping Using the TaqMan® Assay

                John Woodward

7. SNP Genotyping: The KASPTM Assay

                Chunlin He, John Holme, and Jeffrey Anthony

8. Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding

                S. Perez-Elizalde, J. J. Ceron-Rojas, J. Crossa, Delphine Fleury, and G. Alvarado

9. OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs

                Fabio Valente, Franck Gauthier, Nicolas Bardol, Guylaine Blanc, Johann Joets, Alain Charcosset, and Laurence Moreau

10. Genomic Selection in Plant Breeding

                M. A. Newell and J.-L Jannink

11. Simulated Breeding with QU-GENE Graphical User Interface

                Adrian Hathorn, Sctoo Chapman, and Mark Dieters

12. The Control of Recombination in Wheat by Ph1 and Its Use in Breeding

                Graham Moore

13. TILLING for Plant Breeding

                Peter Sharp and Chongmei Dong

14. In vitro Culture for Doubled Haploids: Tools for Molecular Breeding

                Sue Broughton, Parminder K. Sidhu, and Philip A. Davies

15. Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin

                Ainur Ismagul, Gulnur Oskakova, John C. Harris, and Serik Eliby

16. Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)

                Ainur Ismagul, Iryna Mazonka, Corrine Callegari, and Serik Eliby

17. qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number

                Stephen J. Fletcher

18. High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics

                Nataliya Kovalchuk

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts