Date Palm Biotechnology Protocols Volume II, 1st ed. 2017
Germplasm Conservation and Molecular Breeding
Methods in Molecular Biology Series, Vol. 1638

This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology.  Chapters in Date Palm Biotechnology Protocols: Volume 2: Germplasm Conservation and Molecular Breeding guides readers through methods and protocols on germplasm in vitro conservation, molecular analysis of in vitro cultures, genetic diversity,  cultivar identity, gender identification, genomics, and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Date Palm Biotechnology Protocols: Volume 2: Germplasm Conservation and Molecular Breeding aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology.

Part I   Germplasm In Vitro Conservation

Storage and Viability Assessment of Date Palm Pollen

Maryam, Muhammad J. Jaskani, and Summar A. Naqvi

In Vitro Conservation of Date Palm Tissue Cultures

Shawky A. Bekheet

Cryopreservation of Date Palm Pro-embryonic Masses Using the D Cryo-Plate Technique

Mohammad Salma and Florent Engelmann

4                    

Lotfi Fki, Olfa Chkir, Walid Kriaa, Ameni Nasri, Emna Baklouti, Raja B. Masmoudi, Alain Rival, oureddine Drira, and Bart Panis

5                    

Maiada M.  El-Dawayati

6                    

In Vitro Conservation of Date Palm Somatic Embryos Using Growth Retardant Conditions

Mona M. Hassan

7                    

Encapsulation of Date Palm Somatic Embryos: Synthetic Seeds

Shawky A. Bekheet

Part II   Molecular Analysis of In Vitro Cultures

8                    

Evaluation of Clonal Fidelity of Micropropagated Date Palm by Random Amplified Polymorphic DNA (RAPD)

Arpan Modi, Bhavesh Gajera, Naraynan Subhash, and Nitish Kumar

9                    

Molecular Identification of Fungal Contamination in Date Palm Tissue Cultures

Mohammed H. Abass

Part III   Genetic Diversity and Cultivar Identity

Genetic Diversity Analysis of Date Palm Using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Mahesh K. Mahatma, Vishal S. Srivashtav, and Sanjay Jha

11                

Date Palm Genetic Diversity Analysis Using Microsatellite Polymorphism

Hussam S.M. Khierallah, Saleh M. Bader, Alladin Hamwieh, and Michael Baum

12                

Assessing Date Palm Genetic Diversity Using Different Molecular Markers

Mohamed A.M. Atia, Mahmoud M. Sakr, and Sami S. Adawy

13                

Molecular Analysis of Date Palm Genetic Diversity Using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR)

Sherif F. El Sharabasy and Khaled A. Soliman

Determining Phylogenetic Relationships among Date Palm Cultivars Using Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) Markers

Nadia Haider

15                

Genotyping and Molecular Identification of Date Palm Cultivars Using Inter Simple Sequence Repeat (ISSR) Markers

Basim M. Ayesh

16                

Nasser S. Al-Khalifah and A.E. Shanavaskhan

Part IV   Gender Identification

17                

Early Sex Identification in Date Palm by Male Specific Sequence-Characterized Amplified Region (SCAR) markers

Pushpa Kharb and Charu Mitra

18                

Faisal S. Awan, Maryam, Muhammad J. Jaskani, and Bushra Sadia

Development of Sex-Specific PCR-based Markers in Date Palm

Mohamed A.M. Atia, Mahmoud M. Sakr, Morad M. Mokhtar, and Sami S. Adawy

20                

Date Palm Sex Differentiation Based on Fluorescence In Situ Hybridization (FISH)

Mohamed A.M. Atia, Sami S. Adawy, and Hanaiya A. El-Itriby

21                

Characterization and Amplification of Gene-Based Simple Sequence Repeats (SSR) Markers in Date Palm

Yongli Zhao, Manjunath Keremane, Channapatna S. Prakash, and Guohao He

22                

Mitochondrial Molecular Markers for Resistance to Bayoud Disease in Date Palm

Amgad A. Saleh, Anwar H. Sharafaddin, Mahmoud H. El_Komy, Yasser E. Ibrahim, Younis K. Hamad, and Younis Y. Molan

23                

Analysis of Expressed Sequence Tags (EST) in Date Palm

Development of Genomic Simple Sequence Repeats (SSR) by Enrichment Libraries in Date Palm

Sulieman A. Al-Faifi, Hussein M. Migdadi, Salem S. Algamdi, Mohammad Altaf Khan, Rashid S. Al-Obeed, Megahed H. Ammar, and Jerenj Jakse

25                

Wanfei Liu, Chengqi Xin, Jun Yu, and Hasan Awad Aljohi

Part VI   Proteomics

26                

Proteome of Abiotic Stress Tolerance in Date Palm

Proteomics of Development and Germination of Date Palm Zygotic Embryos

Date Fruit Proteomics during Development and Ripening Stages

Claudius Marondedze

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts