Description
G Protein-Coupled Receptor Signaling in Plants, Softcover reprint of the original 1st ed. 2013
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1043
Coordinator: Running Mark P.
Language: EnglishSubjects for G Protein-Coupled Receptor Signaling in Plants:
Publication date: 08-2016
Support: Print on demand
Publication date: 08-2013
162 p. · 17.8x25.4 cm · Hardback
Description
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Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.
1. Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs
Timothy E. Gookin and Jannick D. Bendtsen
2. Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins
Swarup Roy Choudhury, Corey S. Westfall, Dieter Hackenberg, and Sona Pandey
3. Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana
Jian Zhao and Xuemin Wang
4. Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants
Zhaoqing Jin, Wellington Muchero, and Jin-Gui Chen
5. Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis
Kiwamu Tanaka, Jeongmin Choi, and Gary Stacey
6. Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis
Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, and Yiji Xia
7. Fusarium oxysporum Infection Assays in Arabidopsis
Yuri Trusov, David Chakravorty, and Jose Ramon Botella
8. Analysis of Unfolded Protein Response in Arabidopsis
Yani Chen and Federica Brandizzi
9. Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis
Wenjuan Wu and Jirong Huang
10. G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings
Danielle A. Orozco-Nunnelly, Lon S. Kaufman, and Katherine M. Warpeha
11. Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
Xingyun Qi and Huanquan Zheng
12. In Vivo Localization in Arabidopsis Protoplasts and Root Tissue
Myoung Hui Lee, Yongjik Lee, and Inhwan Hwang
13. Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry
Nadav Sorek, Amir Akerman, and Shaul Yalovsky
14. In Vitro Myristoylation Assay of Arabidopsis Proteins
Xuehui Feng, Wan Shi, Xuejun Wang, and Mark P. Running
15. Assaying Protein S-Acylation in Plants
Piers A. Hemsley
16. In Vitro Prenylation Assay of Arabidopsis Proteins
Wan Shi, Qin Zeng, and Mark P. Running
Explores techniques to examine the roles of GCRPs and small GTPases in key plant biological processes
Features step-by-step protocols that are ready for the lab
Includes expert tips and key implementation advice to ensure quality results