Maize, 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1676

This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and plant metabolic networks. The chapters cover a range of topics, such as growing and propagating maize in the laboratory, greenhouse and field studies, screening mutagenic population, characterizing the genome, describing protein and metabolic regulatory networks, and generating transgenic plants for gene knock-out and over expression purposes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Maize: Methods and Protocols is a valuable resource for everyone who is interested in maize research.

Preface…
Table of Contents…
Contributing Authors…

Part I Cell, Tissue, and Organ Culture and Maize Transformation

1    Use of In Vitro Kernel Culture to Study Maize Nitrogen and Carbohydrate Metabolism
Juliann R. Seebauer and Frederick E. Below

2    Agrobacterium- and Biolistic-Mediated Transformation of Maize B104 Inbred
Jennifer A. Raji, Bronwyn Frame, Daniel Little, Tri Joko Santoso, and Kan Wang

3    Advances in Agrobacterium-Mediated Maize Transformation
Heng Zhong, Sivamani Elumalai, Samson Nalapalli, Lee Richbourg, Anna Prairie, David Bradley, Shujie Dong, Jenny Su, Weining Gu, Tim Strebe, Liang Shi, and Qiudeng Que

4    A Brief History of Promoter Development for Use in Transgenic Maize Applications
Part II Gene Silencing and Generation of Mutant Populations

5    Deletion Mutagenesis and Identification of Causative Mutations in Maize
Shangang Jia, Aixia Li, Chi Zhang, and David Holding

6    Selective Knockdowns in Maize by Sequence-Specific Protein Aggregation
Camilla Betti, Joost Schymkowitz, Frederic Rousseau, and Eugenia Russinova

7    Virus-Induced Gene Silencing in Maize with a Foxtail mosaic virus Vector
Yu Mei and Steven A. Whitham

8    Use of a Virus Gene Silencing Vector for Maize Functional Genomics Research
Tao Zhou, Xuedong Liu, and Zaifeng Fan

Part III Plant Gene Expression

9    Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Shanshan Zhang, Dhiraj Thakare, and Ramin Yadegari

10  Ribosome Profiling in Maize
Prakitchai Chotewutmontri, Nicholas Stiffler, Kenneth P. Watkins, and Alice Barkan

11  Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing
Qing Li, Peter J. Hermanson, and Nathan M. Springer

12  Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors
Levi G. Lowder, Aimee Malzahn, and Yiping Qi

13  Cellular and Sub-Cellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging
Maria Emilia Dueñas, Adam D. Feenstra, Andrew R. Korte, Paige Hinners, and Young Jin Lee
         

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts