Description
Photorespiration, Softcover reprint of the original 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1653
Language: EnglishSubject for Photorespiration:
Publication date: 08-2018
Support: Print on demand
Publication date: 08-2017
Support: Print on demand
Description
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This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Estimation of Photorespiratory Fluxes by Gas Exchange
Florian A. Busch, Ross M. Deans, and Meisha-Marika Holloway-Phillips
2. Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling
Amna Mhamdi, Pavel I. Kerchev, Patrick Willems, Graham Noctor, and Frank Van Breusegem
3. Measurement of Enzyme Activities
Hermann Bauwe
4. In vitro Alkylation Methods for Assessing the Protein Redox State
Flavien Zannini, Jérémy Couturier, Olivier Keech, and Nicolas Rouhier
5. Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants
Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J. Boersema, Matthias Mann, and Iris Finkemeier
6. In vitro Analysis of Metabolite Transport Proteins
Marc-Sven Roell, Franziska Kuhnert, Shirin Zamani-Nour, and Andreas P.M. W
eber7. Quantification of Photorespiratory Intermediates by Mass Spectrometry-based Approaches
Stéphanie Arrivault and Toshihiro Obata
8. Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration
Stefan Timm, Katharina Modde, and Hermann Bauwe
9. Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits
Franklin Magnum de Oliveira Silva, Lucas de Ávila Silva, Wagner L. Araújo, Agustin Zsögön, and Adriano Nunes-Nesi
10. Metabolic Engineering of Photorespiration
Martin KM Engqvist and Veronica G Maurino
11. 13CO2 Labelling and Mass Spectral Analysis of Photorespiration
Leonardo Perez de Souza, Marek Szecówka, Alisdair R. Fernie, and Takayuki Tohge
12. Isotopically Nonstationary Metabolic Flux Analysis (INST-M
FA) of Photosynthesis and Photorespiration in PlantsFangfang Ma, Lara J. Jazmin, Jamey D. Young, and Doug K. Allen
13. Genome-scale Modeling of Photorespiratory Pathway Manipulation
Anika Küken and Zoran Nikoloski14. Kinetic Modelling of Photorespiration
Honglong Zhao, Yi Xiao, and Xin-Guang Zhu
15. Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues
Alisdair R. Fernie, Hermann Bauwe, and Lee J. Sweetlove
16. Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana
Stephan Krueger, Ruben M. Benstein, Sabine Wulfert, Armand D. Anoman, María Flores-Tornero, and Roc Ros
17. Light Microscopy, Transmission Electron Microscopy and Immunohistochemistry Protocols for Studying Photorespiration
Roxana Khoshravesh, Vanessa Lundsgaard-Nielsen, Stefanie Sultmanis, and Ta
mmy L SageIncludes cutting-edge and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras