Phototropism, 1st ed. 2019
Methods and Protocols

Methods in Molecular Biology Series, Vol. 1924

Coordinator: Yamamoto Kotaro T.

Language: Anglais

158.24 €

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284 p. · 17.8x25.4 cm · Hardback

This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue and UV lights. They also include methods for auxin biology and analysis of cytoskeleton and phototropin. In addition, the use of grafting, spaceflight experiment and image analysis in tropism study is provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Phototropism: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Measurements of Phototropism


1. Physiological Characterization of Phototropism in Arabidopsis Seedlings

Ken Haga and Taro Kimura


2. Assessing Negative and Positive Phototropism in Lianas

Shogo Kato


3. Determination of Phototropism and Polarotropism in Fern Protonemal Cells

Masamitsu Wada


4. Assays of Protonemal Growth Responses in Physcomitrella patens under Blue- and Red-light Stimuli

Sho Miyazaki, Masatoshi Nakajima, and Hiroshi Kawaide


5. An Experimental System for Examining Phototropic Response of Gametophytic Shoots in the Moss Physcomitrella patens

Liang Bao, Kotaro T. Yamamoto, and Tomomichi Fujita


6. Observation of Phototropic Responses in the Liverwort Marchantia polymorpha

Aino Komatsu, Ryuichi Nishihama, and Takayuki Kohchi


7. Measurement of Phototropism of the Sporangiophore of Phycomycesblakesleeanus

Luis M. Corrochano and Paul Galland


8. Quantitative Analysis of Tip Growth, Phototropic Responses and Other Blue Light-Dependent Photoresponses of Vaucheria

Hironao Kataoka


9. A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezonensis

Megumu Takahashi and Koji Mikami


10. Determination of Phototropism by UV-B Radiation

Lucas Vanhaelewyn, Dominique Van Der Straeten, and Filip Vandenbussche


Part II: Analysis of Auxin

11. New-Generation Chemical Tools for the Manipulation of Auxin Biosynthesis, Action, and Transport

Kosuke Fukui and Ken-ichiro Hayashi


12. Determination of Auxin Flow during Phototropic Responses Using Fluorescent Auxin Analogs

Tatsuya Sakai


13. Immunolocalization of IAA Using an Anti-IAA-C Antibody Raised against Carboxyl-Linked IAA

Takeshi Nishimura and Tomokazu Koshiba


Part III: Miscellaneous Methods

14. Measurements of Photoreaction and Kinase Activity of Phototropin, a Photoreceptor Protein for Tropic Response in Plants; Involvement of Kinase Activity in the Photosensitivity of Tropic Response

Koji Okajima and Satoru Tokutomi


15. Cytoskeleton Observation in Phototropism and Polarotropism of Adiantum Protonema

Masamitsu Wada


16. Grafting as a Tool for Study of Phototropism in Phycomyces

Atsushi Miyazakiand Tamotsu Ootaki


17. Preparation of a Spaceflight Experiment to Study Tropisms in Arabidopsis Seedlings on the International Space Station

Joshua P. Vandenbrink and John Z. Kiss


18. How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening

Haruko Ueda and Ikuko Hara-Nishimura


19. Quantitative Measurements of Curvature along the Growth Axis in Tropic Responses Using Free Software Environments
Kotaro T. Yamamoto and Ken Haga

Includes cutting-edge methods and protocols 

Provides step-by-step detail essential for reproducible results 

Contains key notes and implementation advice from the experts