Phytochromes, 1st ed. 2019
Methods and Protocols

Methods in Molecular Biology Series, Vol. 2026

Coordinator: Hiltbrunner Andreas

Language: Anglais

168.79 €

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290 p. · 17.8x25.4 cm · Hardback

This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phytochromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phytochrome in planta; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate Physcomitrella mutant lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Phytochromes: Methods and Protocols serves as a guideline to researchers who are new to the field, and a stepping stone for experienced researchers looking to expand their knowledge of this evolving field. 

 


Preface…
Table of Contents…
Contributing Authors…

1. Investigation of Light-Regulated Protein-Protein Interactions using Yeast Two-Hybrid Assays
David J. Sheerin

2. Rapid Examination of Phytochrome-PHYTOCHROME INTERACTING FACTOR (PIF) Interaction by In Vitro Co-Immunoprecipitation Assay
Inyup Paik and Enamul Huq

3. Characterization of Light-Regulated Protein-Protein Interactions by In Vivo Co-Immunoprecipitation (Co-IP) Assays from Plant
Ling Zhu and Enamul Huq

4. Detection of Phytochrome Phosphorylation in Plants
Eva Klement, Péter Gyula, and András Viczián

5. Detection of SUMOylated Phytochromes in Plants
Beatriz Orosa and András Viczián

6. Approaches to Identify Protein Ubiquitination Sites in Plants
Feifei Yu and Qi Xie

7. Expression, Purification, and Spectral Characterization of Phytochromes
Yun-Jeong Han, Jae-Yong Cho, and Jeong-Il Kim

8. In Vivo Spectroscopy
Cornelia Klose

9. Basic Phytochrome B Calculations
Robert W. Smith and Christian Fleck

10. Quantitative Analysis of Photobodies
Chan Yul Yoo, Desiree Williams, and Meng Chen

11. Regulation of Alternative Splicing by Phytochrome
Tomonao Matsushita

12. Phytochrome-Regulation of Seed Germination
Kijong Song and Giltsu Choi

13. Shade Avoidance and Neighbor Detection
Irma Roig-Villanova, Sandi Paulišić, and Jaime F. Martinez-Garcia

14. A Simple Method for Quantification of Protochlorophyllide in Etiolated Arabidopsis Seedlings
Matthew J. Terry and Sylwia M. Kacprzak

15. Measuring Phytochrome-Dependant Light-Input to the Plant Circadian Clock
Rachael J. Oakenfull, James Ronald, and Seth J. Davis

16. Characterization of Flowering Time Mutants
Xu Wang, Qing Liu, Wenjin He, Chentao Lin, and Qin Wang

17. Rapid Detection of Hormonal Involvement in Light Responses
Filip Vandenbussche

18. Phytochrome and Light Signaling in Marchantia
Keisuke Inoue, Ryuichi Nishihama, and Takayuki Kohchi

19. Analysis of Physcomitrella Phytochrome Mutants via Photo- and Polarotropism
Anna Lena Ermert, Fabian Stahl, Tanja Gans, and Jon Hughes

20. CRISPR/Cas9-Mediated Knockout of Physcomitrella patens Phytochromes
Anna Lena Ermert, Fabien Nogué, Fabian Stahl, Tanja Gans, and Jon Hughes

21. Phylogenetic Methods to Study Light Signaling
Fay-Wei Li and Sarah Mathews

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts