Plant Genome Editing with CRISPR Systems, 1st ed. 2019
Methods and Protocols

Methods in Molecular Biology Series, Vol. 1917

Coordinator: Qi Yiping

Language: Anglais
Cover of the book  Plant Genome Editing with CRISPR Systems

Subject for Plant Genome Editing with CRISPR Systems

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367 p. · 17.8x25.4 cm · Hardback

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Table of Contents…
Contributing Authors…

Part I Review on Plant DNA Repair and Genome Editing
1. Plant DNA Repair Pathways and their Applications in Genome Editing
Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, and Mary-Dell Chilton 

Part II CRISPR Design and Mutation Analysis

2. Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method
Chun Wang and Kejian Wang

3. Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations
Xianrong Xie, Xingliang Ma, and Yao-Guang Liu

Part III Multiplexed CRISPR-Cas9 Systems 

4. Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9
Riqing Li, Si Nian Char, and Bing Yang

5. A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing
Kabin Xie and Yinong Yang

6. A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants
Xu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, and Yong Zhang

7. CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation
Aimee Malzahn, Yong Zhang, and Yiping Qi

Part IV CRISPR-Cas9 Editing in Monocots

8. Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9
Weihang Gu, Dabing Zhang, Yiping Qi, and Zheng Yuan

9. Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9
Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Mengqiao Yang, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang

10. An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize
Keunsub Lee, Huilan Zhu, Bing Yang, and Kan Wang

Part V CRISPR-Cas9 Editing in Dicots

11. Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants
Hanchuanzhi Yu and Yunde Zhao

12. Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9
Tom Lawrenson, Penny Hundleby, and Wendy Harwood

13. Application of CRISPR/Cas9-Mediated Gene Editing in Tomato
Nathan T. Reem and Joyce Van Eck

14. Genome Editing in Potato with CRISPR/Cas9
Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, and David S. Douches

15. Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells
Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, and Rafal Baranski

16. Genome Editing in Soybean with CRISPR/Cas9
Junqi Liu, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, and Robert M. Stupar

17. Genome Editing in Citrus Tree with CRISPR/Cas9
Hongge Jia, Xiuping Zou, Vladimir Orbovic, and Nian Wang

Part VI CRISPR-Cas12a Editing Systems

18. Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems
Yingxiao Zhang, Yong Zhang, and Yiping Qi

19. Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1
Abhishek Anand, Xiaojia Yin, Paul Quick, and Anindya Bandyopadhyay

20. Targeted Mutagenesis using FnCpf1 in Tobacco
Akira Endo and Seiichi Toki

Part VII Precise Gene Editing

21. Gene Replacement by Intron Targeting with CRISPR-Cas9
Jun Li, Xiangbing Meng, Jiayang Li, and Caixia Gao

22. Targeted Base Editing with CRISPR-Deaminase in Tomato
Zenpei Shimatani, Tohru Ariizumi, Ushio Fujikura, Akihiko Kondo, Hiroshi Ezura, and Keiji Nishida

Part VIII Non-Agrobacterium Based CRISPR Delivery Systems

23. Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System
Ahmed Mahas, Zahir Ali, Manal Tashkandi, and Magdy M. Mahfouz

24. Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat
Zhen Liang, Kunling Chen, and Caixia Gao

25. DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce
Jongjin Park, Aiden Y. Park, Slki Park, Jiyoung Yoon, Sunmee Choi, and Sunghwa Choe

26. An Automated Protoplast Transformation System
Scott C. Lenaghan and C. Neal Stewart, Jr.
Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts