Transgenic Cotton (2^nd Ed., 2nd ed. 2019)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1902

This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detail current status and perspectives of transgenic cotton, principle and methods for making transgenic cotton, creating gene knockout lines, foreign gene copy and expression in transgenic plants, improvements to cotton using transgenic technology, and monitoring the potential impact of transgenic cotton on environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and cutting-edge, Transgenic Cotton: Methods and Protocols 2^nd aims to be a resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology, and agricultural sciences.

Part I: Introduction

1. Transgenic Cotton: From Biotransformation Methods to Agricultural Application

Baohong Zhang

Part II: Transformation

2. Agrobacterium-mediated Genetic Transformation of Cotton

Baohong Zhang

3. Biolistic Transformation of Cotton Zygotic Embryo Meristem

K. Rajasekaran

4. Novel Pollen Magnetofection System for Transformation of Cotton Plant with Magnetic Nanoparticles as Gene Carriers

Rui Zhang, Zhigang Meng_, Muhammad Ali Abid, and Xiang Zhao

5. Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures

K. Rajasekaran

6. Pollen Tube Pathway-Mediated Cotton Transformation

Min Wang, Runrun Sun, Baohong Zhang, and Qinglian Wang

7. Embryogenic Calli Explants and Silicon Carbide Whisker-mediated Transformation of Cotton (Gossypium hirsutum L.)

Part III: Genome Editing and Vigs

8. Genome Editing in Cotton Using CRISPR/Cas9 System

Chao Li and Baohong Zhang

9. Tobacco Rattle Virus Induced Gene Silencing in Cotton

Caiping Cai, Xinyu Wang, Baohong Zhang, and Wangzhen Guo

10. Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome

Jun Zhang and Yan Hong

11. Estimating the Copy Number of Transgenes in Transformed Cotton by Real-time Quantitative PCR

Chengxin Yi and Yan Hong

12. Development of an Enzyme-linked Immunosorbant (ELISA) Assay for the Detection of GMProtein in GM Crops/Produce

Suchitra Kamle, Dawei Li, Abhishek Ojha, and Arvind Kumar

13. Screening of Transgenic Cotton Based on a Porous Silicon Biosensor 

Liangliang Chen, Peng Li, Xiaoyi Lv, and Ji Ma

14. YC3.60-based Imaging Analysis on Calcium Level in Cotton Cells

Mi Zhang, Jianyan Zeng, Huizhen Cao, and Yan Pei

15. A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene

16. A Grafting Technique for Efficiently Transplanting Transgenic Regenerated Plants of Cotton

Min Wang, Qinglian Wang, and Baohong Zhang

17. Inheritance of Transgenes in Transgenic Bt lines Resistance to Helicoerpa armigera in Upland Cotton

Baolong Zhang, Wangzhen Guo, and Tianzhen Zhan

Part V: Application

18. Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics

Hee Jin Kim

19. Overexpression of miRNA in Cotton via Agrobacterium-mediated Transformation

Min Wang, Runrun Sun, Qinglian Wang, and Baohong Zhang

20. Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover

Zhi Liu, Zhen Zhu, and Tianzhen Zhang

21. Next Generation Transgenic Cotton: Pyramiding RNAi with Bt Counters Insect Resistance

Wei Ma and Tianzhen Zhang

22. Genetic Transformation of Cotton with the Harpin-encoding Gene hpa_Xoo of Xanthomonas oryzae pv. Oryzae and Evaluation of Resistance against Verticillium Wilt

Weiguo Miao and Jingsheng Wang

23. Development of Insect-resistant Transgenic Cotton with Chimeric TVip3A* Accumulating in

Chloroplasts

JiaheWu and Yingchuan Tian

24. Development of Virus Resistance Transgenic Cotton using Cotton Leaf Curl Virus Antisense ßC1 Gene

S.S. Sohrab

Part VI:  Risk Assessment

25. Determining Pollen-mediated Gene Flow in Transgenic Cotton

Xiaoping Pan

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts