Xylem, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1544

This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue.  Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.

Part I: DEVELOPMENT

1 Experimental and Theoretical Methods to Investigate Vascular Development in Plants

2 Strigolactone-Mediated Stimulation of Secondary Xylem Proliferation in Stems

Javier Agustí

3 Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues

Sacha Escamez, Benjamin Bollhöner, and Hannele Tuominen

4 Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis

Delphine Ménard, Henrik Serk, Raphaël Decou, and Edouard Pesquet

5 Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

Masato Saito, Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda

6 VND6-Induced Xylem Cell Differentiation in Arabidopsis Cell Cultures

Oda Yoshida

Part II: IMAGING

7 Live Imaging of Developing Xylem in Planta

Raymond Wightman

8 Immunolocalization in Secondary Xylem of Populus

Suzanne Gerttulaand Andrew Groover

9 Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis Thaliana

Charles Melnyk

10 Vascular Morphodynamics during Secondary Growth

Pierre Barbier De Reuille and Laura Ragni

11 Improved Mps-PI Staining for Xylem Characterization

Elisabeth Truernit

12 Chemical Imaging of Xylem by Raman Microspectroscopy

Andras Gorzsas

13 Using Cellprofiler to Analyse and Quantify Vascular Morphology

Liam Campbell, Manoj Kumar, and Simon Turner

Part III: COMPOSITION

14 Lignin Analysis by HPLC and FTIR

15 Carbohydrate Composition Analysis in Xylem

Baocai Zhang and Yihua Zhou

16  Structural Analysis of Cell Wall Polysaccharides Using PACE

Jennifer C. Mortimer

17 Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy

Raphaël Decou, Henrik Serk, Delphine Ménard, and Edouard Pesquet

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras