Description
Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis, Softcover reprint of the original 1st ed. 2014
Springer Theses Series
Author: Wang Fangjun
Language: EnglishSubject for Applications of Monolithic Column and Isotope...:
Keywords
Isotope Dimethylation Labeling; Mass Spectrometry; Multidimensional Chromatography Separation; Polymer-Based Hydrophobic Monolithic Column; Protein Phosphorylation; Proteome Analysis; Quantitative Proteomics; Separation of Complex Peptide Mixtures; Stable Isotope Labeling; Strong Cation-Exchange Monolithic Column; chromatography; Proteomics
Approximative price 105.49 €
In Print (Delivery period: 15 days).
Add to cart the print on demand of Wang FangjunPublication date: 08-2016
Support: Print on demand
Approximative price 105.49 €
In Print (Delivery period: 15 days).
Add to cart the book of Wang FangjunPublication date: 12-2013
94 p. · 15.5x23.5 cm · Hardback
Description
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In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer?s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.
Nominated by Chinese Academy of Sciences as an outstanding PhD thesis
Describes the first example of online multidimensional chromatography separation using capillary monolithic columns
Introduces the combination of online stable isotope labeling and multidimensional chromatography separation
Outlines the proteome and phosphoproteome expression profiles of clinical hepatocellular carcinoma tissues and hippocampi of mice with Alzheimer’s disease
Includes supplementary material: sn.pub/extras