Mass Spectrometry in Metabolomics, 2014
Methods and Protocols

Methods in Molecular Biology Series, Vol. 1198

Coordinator: Raftery Daniel

Language: Anglais

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Mass Spectrometry in Metabolomics
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Mass Spectrometry in Metabolomics. Methods and Protocols
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360 p. · 17.8x25.4 cm · Hardback

Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples, and this information provides enormous detail on biological systems and their current status. Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance, and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.

Part I. Historical and Methodology Overview

1. Overview: of Mass Spectrometry Based Metabolomics: Opportunities and Challenges

Part II. Global LC-MS Based Metabolic Profiling

2. Global Metabolic Profiling using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry

3. LC-MS Profiling to Link Metabolic and Phenotypic Diversity in Plant Mapping Populations

4. Mitochondrial Metabolomics Using High-Resolution Fourier-Transform Mass Spectrometry

5. Sample Preparation Methods for LC-MS Based Global Aqueous Metabolite Profiling

Part III. Global GC-MS and CE-MS Based Metabolic Profiling

6. Methods of Discovery-Based and Targeted Metabolite Analysis by Comprehensive Two-Dimensional Gas Chromatography with Time-of-Flight Mass Spectrometry Detection

7. Analysis of Mouse Liver Metabolites by GC×GC–TOF MS

8. Metabolite Fingerprinting by Capillary Electrophoresis-Mass Spectrometry

Part IV. Isotope Labeling Methods

9. Quantitative Metabolomic Profiling using Dansylation Isotope Labeling and Liquid Chromatography Mass Spectrometry

10. Quantitative Analysis of Amino and Organic Acids by Methyl Chloroformate Derivatization and GC-MS/MS Analysis

11. Stable Isotope Labeled Tracers for Metabolic Pathway Elucidation by GC-MS and FT-MS

Part V. Targeted Aqueous Metabolite Profiling

12. Multiplexed, Quantitative and Targeted Metabolite Profiling by LC-MS/MRM

Part VI. Lipid Profiling Methods

13. Multi-Dimensional Mass Spectrometry-Based Shotgun Lipidomics

14. Comprehensive Quantitative Determination of PUFA-Related Bioactive Lipids for Functional Lipidomics using High Resolution Mass Spectrometry

15. Ultra-Performance Liquid Chromatography Mass Spectrometry Targeted Profiling of Bile Acids: Application to Serum, Liver Tissue and Cultured Cells of Different Species

Part VII. Profiling Volatile Organic Compounds

16. Analysis of Volatile Organic Compounds in Exhaled Breath by Gas Chromatography-Mass Spectrometry combined with Chemometric Analysis

17. Headspace SPME-GC-MS Metabolomics Analysis of Urinary Volatile Organic Compounds (VOCs)

Part VIII. Profiling and Imaging using Atmospheric Sampling Methods

18. Metabolite Profiling by Direct Analysis in Real Time Mass Spectrometry

19. Analysis of Dried Blood Spots using DESI Mass Spectrometry

20. DESI-MS Imaging of Lipids and Metabolites from Biological Samples

21. Metabolic Imaging using Nanostructure-Initiator Mass Spectrometry (NIMS)

Part IX. Statistical Analysis Methods

22. Statistical Analysis and Modeling of Mass Spectrometry-Based Metabolomics Data

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts