SELDI-TOF Mass Spectrometry, Softcover reprint of the original 1st ed. 2012
Methods and Protocols
Methods in Molecular Biology Series, Vol. 818

SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization in diverse applications including diagnostics, drug development, and basic research. SELDI-based biomarker studies can typically be divided into four phases: discovery, validation, purification and identification, and assay development. SELDI-TOF Mass Spectrometry: Methods and Protocols provides an overview of the current applications of SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry), with an emphasis on study and experimental design, data analysis and interpretation, and assay development. Written in the highly successful Methods in Molecular Biology? series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, SELDI-TOF Mass Spectrometry: Methods and Protocols will provide information on optimizing study design, experimental protocols, and data analysis and interpretation to yield robust biomarkers and biomarker assays, using examples from different disease areas.

1. Optimized Conditions for a Quantitative SELDI TOF MS Protein Assay

            Lee Lomas, Charlotte Clarke, Vanitha Thulasiraman, and Eric Fung

2. Solid-phase Fractionation Strategies Applied to Proteomics Investigations

            Luc Guerrier, Frederic Fortis, and Egisto Boschetti

3. Data Processing and Analysis Using ProteinChip® Data Manager Software

            Enrique A. Dalmasso and Dominic Caseñas

4. Purification and Identification of Candidate biomarkers discovered using SELDI TOF MS

            Amanda L. Bulman and Enrique Dalmasso

5. Biomarker Discovery in Serum/Plasma Using Surface Enhanced Laser Desorption Ionization Time Of Flight (SELDI TOF) Mass Spectrometry Platform

            Momar Ndao

6. Plasma Proteomic Profiling of Pediatric Osteosarcoma

            Yiting Li, TuAhn Dang, and Tsz-Kwong Man

7. Profiling of Urine Using ProteinChip Technology

            Ronald L. Woodbury, Diane B. McCarthy, and Amanda L. Bulman

8. Protein Profiling of Cerebrospinal Fluid

            Anja H. Simonsen

9. SELDI TOF Mass Spectrometry-Based Protein Profiling of Tissue Samples for Toxicological Studies

            Alexandra Sposny and Philip G. Hewitt

10. Proteomic Analysis of Skeletal Muscle Tissue Using SELDI TOF MS: Application to Disuse Atrophy

            Mark S. F. Clarke

11. Profiling Cervical Lavage Fluid by SELDI-TOF Mass Spectrometry          

            Adam Burgener

12. Isolation and Proteomic Analysis of Platelets by SELDI TOF MS

            Sean R. Downing and Giannoula L. Klement

13. Using SELDI-TOF Mass spectrometry on Amniotic Fluid and for Clinical Proteomics and Theranostics in Disorders of Pregnancy

            Irina A. Buhimschi

14. High Throughput Profiling of Serum Phosphoproteins/ peptides using the SELDI TOF MS Platform

            Lin Ji, Gitanjali Jayachandran and Jack A. Roth

15. Analysis of Protein-protein Interaction using ProteinChip Array- based SELDI TOF Mass Spectrometry

            Gitanjali Jayachandran, Jack A. Roth, and Lin Ji

16. Quantitation of Amyloid Beta Peptides in CSF by Surface Enhanced MALDI-TOF

            Eddie Takahashi, Anita Howe, Ole Vesterqvist and Zhaosheng Lin

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras