Bacterial Virulence, 1st ed. 2022
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2427

Coordinator: Gal-Mor Ohad

Language: English

126.59 €

In Print (Delivery period: 15 days).

Add to cartAdd to cart
Bacterial Virulence
Publication date:
267 p. · 17.8x25.4 cm · Paperback

179.34 €

In Print (Delivery period: 15 days).

Add to cartAdd to cart
Bacterial Virulence
Publication date:
267 p. · 17.8x25.4 cm · Hardback
This detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence.

Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Part I: Molecular Biology and Bioinformatics Methods

 

1. Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection

            Shai Ran Sapir, Etai Boichis, and Anat A. Herskovits

 

2. A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria

            Daniel Tchelet and Dor Salomon

 

3. Predicting Type III Effector Proteins Using the Effectidor Web-Server

            Naama Wagner, Doron Teper, and Tal Pupko

 

4. Assay for Type III Secretion in Escherichia coli

            Bosko Mitrovic and Neta Sal-Man

 

5. Profiling of Secreted Type 3 Secretion System Substrates by Salmonellaenterica

            Rivka Shem-Tov and Ohad Gal-Mor

 

Part II: Cell Culture and Organoid Models of Infection

 

6. Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay

            Sara Vilela Pais, Sibel Westerhausen, Erwin Bohn, and Samuel Wagner

 

7. Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility

            Leah Rankine-Wilson, Céline Rens, Henok Asfaw Sahile, and Yossef Av-Gay

 

8. Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes

            Etai Boichis, Shai Ran Sapir, and Anat A. Herskovits

 

9. Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages

            Izumi Sasaki and Tsuneyasu Kaisho

 

10. Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments

            Marc Schulte and Michael Hensel

 

11. Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections

            Erez Mills and Erik Petersen

 

12. Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing

            Shelly Hen-Avivi and Roi Avraham

 

13. Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers

            Erin C. Boyle, Eva J. Wunschel, and Guntram A. Grassl

 

Part III: In Vivo Models of Infection

 

14. Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce

            Laura Elpers and Michael Hensel

 

15. Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity

            Ling Ning Lam, Debra N. Brunson, Jessica K. Kajfasz, and José A. Lemos

 

16. Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

            Miriam Ravins, Poornima Ambalavanan, Debabrata Biswas, Rachel Tan Ying Min, Kimberly Lim Xuan Zhen, Yael Kaufman, Aparna Anand, Abhinay Sharma, and Emanuel Hanski

 

17. Mouse Model to Study Salmonella-Induced Colitis

            Katrin Ehrhardt and Guntram A. Grassl

 

18. Analysis of SalmonellaTyphi Pathogenesis in a Humanized Mouse Model

            Taylor A. Stepien, Stephen J. Libby, Joyce E. Karlinsey, Michael A. Brehm, Dale L. Greiner, Leonard D. Shultz, Thea Brabb, and Ferric C. Fang

 

19. In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella

            Michaël Koczerka, Isabelle Lantier, Anne Pinard, Marie Morillon, Justine Deperne, Ohad Gal-Mor, Olivier Grépinet, and Isabelle Virlogeux-Payant

 

20. Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks

            Philippe Velge, Pierrette Menanteau, Thierry Chaumeil, Emilie Barilleau, Jérôme Trotereau, and Isabelle Virlogeux-Payant

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts