Enzyme Activity in Single Cells

Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more.

1. Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome Songon An, Miji Jeon, Erin L. Kennedy and Minjoung Kyoung 2. Synthetic probe development for measuring single or few-cell activity and efflux Alison Lui, Jeffrey Wang, Linda Chio and Markita P. Landry 3. Enzymatic activity in single cells Josephine Geertsen Keller, Magnus Stougaard and Birgitta R. Knudsen 4. Single-cell assays using integrated continuous-flow microfluidics Ee Xien Ng, Myat Noe Hsu, Guoyun Sun and Chia-Hung Chen 5. Single-cell activity screening in microfluidic droplets Stefanie Neun, Tomasz S. Kaminski and Florian Hollfelder 6. The microfluidic capture of single breast cancer cells for multi-drug resistance assays Karan Parekh, Hamide Sharifi, Avid Khamenehfar, Timothy V. Beischlag, Robert T.M. Payer and Paul C.H. Li 7. Single-cell proteolytic activity measurement using microfluidics for rare cell populations Yu-Chih Chen and Euisik Yoon 8. Microfluidic technology for investigation of protein function in single adherent cells Aldo Jesorka, Inga Põldsalu and Irep Gözen 9. Nanokits for the electrochemical quantification of enzyme activity in single living cells Rongrong Pan and Dechen Jiang 10. Design of an automated capillary electrophoresis platform for single-cell analysis David H. Abraham, Matthew M. Anttila, Luke A. Gallion, Brae V. Petersen, Angela Proctor and Nancy L. Allbritton 11. Microelectrophoretic single-cell measurements with microfluidic devices Jay Sibbitts, Jalal Sadeghi and Christopher T. Culbertson 12. Chemical probes for spatially resolved measurement of active enzymes in single cells Gang Li and Raymond E. Moellering 13. Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry Camille Lombard-Banek, Sam B. Choi and Peter Nemes 14. Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells Ruichuan Yin, Venkateshkumar Prabhakaran and Julia Laskin

This book will be of interest to the cell biologist focused on measuring cellular physiology at the single-cell level as well as scientists and engineers seeking to develop highly sensitive technologies for cutting edge cell-based assays.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
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