Enzyme-Mediated Ligation Methods, 1st ed. 2019
Methods in Molecular Biology Series, Vol. 2012

Coordinators: Nuijens Timo, Schmidt Marcel

Language: Anglais

137.14 €

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385 p. · 17.8x25.4 cm · Hardback

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. 

Table of Contents…
Contributing Authors…

1. Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates
Rémy Gébleux, Manfred Briendl, Ulf Grawunder, and Roger R. Beerli

2. Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)
Hejia Henry Wang and Andrew Tsourkas

3. Cyclizing Disulfide-Rich Peptides using Sortase A
Akello J. Agwa, David J. Craik, and Christina I. Schroeder

4. Chemo-Enzymatic Synthesis of  Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1
Marcel Schmidt and Timo Nuijens

5. Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE)
Igor Rupniewski and David Rabuka

6. Butelase 1-Mediated Ligation of Peptides and Proteins
Xinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, and James P. Tam

7. Trypsiligase-Catalyzed Peptide and Protein Ligation
Sandra Liebscher and Frank Bordusa

8. Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase
Stephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, and Nicolas Rasche

9. Tailoring Activity and Selectivity of Microbial Transglutaminase 
Lukas Deweid, Olga Avrutina, and Harald Kolmar

10. SpyLigase-Catalyzed Modification of Antibodies
Vanessa Siegmund, Birgit Piater, Frank Fischer, and Harald Kolmar

11. Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases
Wael E. Houssen

12. In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis
Karen S. Harris, Simon Poon, Pedro Quimbar, and Marilyn A. Anderson

13. Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation
Jan Grünewald, Ansgar Brock, and Bernhard H. Geierstanger

14. Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization
Joseph G. Plaks and Joel L. Kaar

15. BioID as a Tool for Protein Proximity Labeling in Living Cells
Rhiannon M. Sears, Danielle G. May, and Kyle J. Roux

16. N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells
Samuel H. Ho and David A. Tirrell

17. Tubulin Tyrosine Ligase-Mediated Modification of Proteins
Marcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P.R. Hackenberger, Dominik Schumacher, and Jonas Helma

18. Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine
Jorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, and Floria L. van Delft

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts