Enzyme Stabilization and Immobilization (2^nd Ed., Softcover reprint of the original 2nd ed. 2017)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1504

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

1. Introduction to the Field of Enzyme Immobilization and Stabilization
Michael J. Moehlenbrock and Shelley D. Minteer

2. Stabilization of Enzymes through Encapsulation in Liposomes
Makoto Yoshimoto

3. Micellar Enzymology for Thermal, pH, and Solvent Stability
Shelley D. Minteer

4. Lipase Activation and Stabilization in Room Temperature Ionic Liquids
Joel L. Kaar

5. Nanoporous Gold for Enzyme Immobilization
Keith J. Stine, Kenise Jefferson, and Olga V. Shulga

6. Enzyme Stabilization via Bio-Templated Silicification Reactions
Glenn R. Johnson and Heather R. Luckarift

7. Covalent Immobilization of Enzymes on Eupergit^® Supports: Effect of the Immobilization Protocol
Zorica D. Knežević-Jugović, Sanja Ž. Grbavčić, Jelena R. Jovanović, Andrea B. Stefanović, Dejan I. Bezbradica, Dušan Ž. Mijin, and Mirjana G. Antov

8. Micellar Polymer Encapsulation of Enzymes
Sabina Besic and Shelley D. Minteer

9. Cross-Linked Enzyme Aggregates for Applications in Aqueous and Non-Aqueous Media
Ipsita Roy, Joyeeta Mukherjee, and Munishwar N. Gupta

10. Protein Coated Microcrystals, Combi-Protein Coated Microcrystals and Cross-Linked Protein Coated Microcrystals of Enzymes for Use in Low Water Media
Joyeeta Mukherjee and Munishwar N. Gupta

11. Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization
Nenad B. Milosavić, Radivoje M. Prodanović, Dušan Veličković, and Aleksandra Dimitrijević

12. Cytochrome C Stabilization and Immobilization in Aerogels
Amanda S. Harper-Leatherman, Jean Marie Wallace, and Debra R. Rolison

13. Enzyme Immobilization and Mediation with Osmium Redox Polymers
Gaige R. VandeZande, Jasmine M. Olvany, Julia L. Rutherford, and Michelle Rasmussen

14. Ferrocene-Modified Linear Poly(ethylenimine) for Enzymatic Immobilization and Electron Mediation
David P. Hickey

15. FAD-Dependent Glucose Dehydrogenase Immobilization and Mediation within a Naphthoquinone Redox Polymer
Ross D. Milton

16. Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes
Alice H. Suroviec

17. Kinetic Measurement for Enzyme Immobilization
Michael J. Cooney

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras