Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding
Methods in Enzymology Series, Vol. 583

Language: Anglais
Cover of the book Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

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Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.



  • Covers a subset of enzymes that work in the environment of the biological cell membrane
  • Offers a series of experimental approaches for the isolation and study of enzymes
  1. A High-Throughput Flurometric Assay for Lipid-Protein Binding
    Wonhwa Cho
  2. Fluorescence-based in situ quantitative imaging for cellular lipids
    Wonhwa Cho
  3. Recombinant Expression of human and mouse groups I, II, III, V, V and XII Secreted Phospholipases A2
    Michael H. Gelb and Gerard Lambeau
  4. Vesicle-based assay of secreted phospholipase A2 enzymatic activity for high throughput screening of compound libraries
    Michael H. Gelb
  5. Cellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2a to Membrane
    Christina C. Leslie
  6. Secreted Phospholipase A2 Specificity on Natural Membrane Phospholipids
    Makoto Murakami
  7. Analyses of Calcium-Independent Phospholipase A2beta (iPLA2ß) in Biological Systems
    Sasanka Ramanadham
  8. Using hydrogen deuterium exchange mass spectrometry to examine protein membrane interactions
    John E. Burke
  9. Lipid-binding C2 domains mediate a diverse class of enzymatic functions"
    Robert V. Stahelin
  10. Interfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and Activity
    Suren Tatulian
  11. Enzymology at the Membrane Interface
    Dan M. Raben
  12. Studying gastric lipase adsorption onto phospholipid monolayers by surface tensiometry, ellipsometry and atomic force microscopy
    Frédéric Carrière
  13. Probing conformational changes and interfacial recognition site of lipases with surfactants and inhibitors
    Frédéric Carrière
  14. Measuring Phospholipase D enzymatic activity through biochemical and imaging methods
    Michael A. Frohman
  15. Pld Rotein-Protein Interaction with Intracellular Signaling Molecules, Modulation by Mitogen Pa and Pld’s Gef Activity
    Julian G. Cambronero
  16. Phosphatidic Acid Regulation of PIPKI
    Guangwei Du
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists