Functional Disulphide Bonds, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1967

Coordinator: Hogg Philip

Language: English

158.24 €

Subject to availability at the publisher.

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327 p. · 17.8x25.4 cm · Hardback
This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study.
Chapter 1: Introduction and overview

Chapter 2: Structural disulphide bonds: review

Chapter 3: Allosteric disulphide bonds: review

Chapter 4: Catalytic disulphide bonds: review

Chapter 5: Evolution of disulphide bonds

Chapter 6: Molecular dynamics of disulphide bonds

Chapter 7: Structural biology of disulphide bonds

Chapter 8: Bioinformatics of functional disulphides #1

Chapter 9: Bioinformatics of functional disulphides #2

Chapter 10: Mechano-chemistry of disulphide bonds

Chapter 11: Oxidoreductase cleavage of disulphide bonds

Chapter 12: Thiol-disulphide cleavage of disulphide bonds

Chapter 13: Experimental screens for identification of functional disulphides

Chapter 14: Quantification of the redox state of functional disulphides

Chapter 15: Kinetic trapping of allosteric disulphides

Chapter 16: Identification of allosteric disulphides from labile disulphides in protein structures

Chapter 17: S-nitrosylation control of functional disulphides

Chapter 18: S-glutathionylation control of functional disulphides

Chapter 19: Reporter probes of functional disulphide bonds

Chapter 20: Measurement of subcellular redox potentials

Chapter 21: Oxidoreductase inhibitors

Chapter 22: Case study: disulphide control of transglutaminase 2

Chapter 23: Case study: disulphide control of Lon protease

Chapter 24: Case study: disulphide control of CD4 immune function

Chapter 25: Case study: disulphide control of protein kinases

Chapter 26: Case study: disulphide control of integrin function

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts