Histochemistry of Single Molecules, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1560

Coordinators: Pellicciari Carlo, Biggiogera Marco

Language: English

158,24 €

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

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This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets.  It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA  fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.

Part I: Vital Histochemistry

 

1. Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes

            Anna L. Peters and Cornelis J.F. van Noorden

 

2. Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues

            Anna C. Croce and Giovanni Bottiroli

 

3. Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

            Vittorio Bertone, Eleonora Tarantola, and Isabel Freitas

 

4. Histochemical Demonstration of Tripeptidyl Aminopeptidase I

            Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, and Nikolai E. Lazarov

 

5. Enzyme Histochemistry for Functional Histology in Invertebrates

            Francesca Cima

 

Part II: Lectin Histochemistry

 

6. Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future

            Susan A. Brooks

 

7. Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads

            Ellie-May Beaman, David R.F. Carter, and Susan A. Brooks

 

8. Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells

            Gerrit Wolters-Eisfeld and Udo Schumacher

 

9. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

            Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Hernández-Martínez, Concepción Ferrer, and Luis Miguel Pastor

 

Part III: Protein Histochemistry

 

10. Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications

            Shuji Yamashita and Osamu Katsumata

 

11. Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain

            Sara Bonzano and Silvia De Marchis

 

12. Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration

            Nicolas A. Dumont and Michael A. Rudnicki

 

13. Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue

            Sabina Berezowska and José A. Galván

 

Part IV: Lipid Histochemistry

 

14. Tissue Fixation and Processing for the Histological Identification of Lipids

            Víctor Carriel, Fernando Campos, José Aneiros-Fernández, and John A. Kiernan

 

15. Staining Methods for Normal and Regenerative Myelin in the Nervous System

            Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, and Stefano Geuna

 

16. Nile Red Staining of Neutral Lipids in Yeast

            Kerry Ann Rostron and Clare Louise Lawrence

 

17. Staining of Lipid Droplets with Monodansylpentane

            Bo-Hua Chen, Huei-Jiun Yang, He-Yen Chou, Guang-Chao Chen, and Wei Yuan Yang

 

Part V: Nuclear Histochemistry

 

18. Fluorochromes for DNA Staining and Quantitation

            Giuliano Mazzini and Marco Danova

 

19. Osmium Ammine for Staining DNA at Electron Microscopy

            Irene Masiello and Marco Biggiogera

 

20. DNA Labeling at Electron Microscopy

            Nicolas Thelen and Marc Thiry

 

21. Visualizing RNA at Electron Microscopy by Terbium Citrate

            Marco Biggiogera and Irene Masiello

 

22. Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks

            Elva I. Cortés-Gutiérrez, José Luis Fernández, Martha I. Dávila-Rodríguez, Carmen López-Fernández, and Jaime Gosálvez

 

Part VI: Plant Histochemistry

 

23. Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins

            Malgorzata Ciska and Susana Moreno Díaz de la Espina

 

24. Histochemical Analysis of Plant Secretory Structures

            Diego Demarco

 

25. A Histochemical Technique for the Detection of Annonaceous Acetogenins

            Guillermo Laguna-Hernández, Alicia Enriqueta Brechú-Franco, Iván De la Cruz-Chacón, and Alma Rosa González- Esquinca

Includes cutting-edge techniques for the study of single molecule histochemistry

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts