Hnasenula polymorpha : Biology and Applications

Methylotrophic yeasts have attracted increasing interest as useful systems for fundamental research and applied purposes. Hansenula polymorpha in particular has become a preferred organism for the production of recombinant proteins on an industrial scale. Product examples range from therapeutics such as hepatitis B vaccines to industrial enzymes like the feed additive phytase. This book is addressed to researchers and scientists working in the field and provides a comprehensive, up-to-date overview of the present status of Hansenula polymorpha research, applications and methods. Aspects of the organism ranging from systematics, genetics, methanol metabolism and peroxisomal function to its use as a technology platform for the production of recombinant proteins are covered. A detailed chapter on laboratory methods is also included.

History, habitat, variability, nomeclature and phylogenetic position of Hansenula polymorpha
Basic genetics of Hansenula polymorpha
Biochemistry and genetics of nitrate assimilation
Amino acid biosynthesis
Methanol metabolism
Hansenula polymorpha: a versatile model organism in peroxisome research
Characteristics of the Hansenula polymorpha genome
The expression platform based on Hansenula polymorpha strain RB11 and its derivatives history, status and perspectives
Development of expression systems for the production of recombinant proteins in Hansenula polymorpha DL 1
Foreign gene expression in Hansenula polymorpha approaches for "difficult proteins"
Fermentation and primary product recovery
Recombinant hepatitis B vaccines disease characterization and vaccine production
Production of anticoagulants in Hansenula polymorpha
Production of cytokines in Hansenula polymorpha
Technical enzyme production and whole cell biocatalysis: application of Hansenula polymorpha
Biosafety aspects of genetically engineered Hansenula polymorpha a case study about non deliberate environmental releases
Methods
Appendix