Description
Macrophages, Softcover reprint of the original 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1784
Coordinator: Rousselet Germain
Language: EnglishSubject for Macrophages:
Publication date: 12-2019
278 p. · 17.8x25.4 cm · Paperback
Support: Print on demand
Description
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Preface…
Table of Contents…
Contributing Authors…
1. Culture of Human Monocyte-Derived Macrophages
Aoife Kelly, Aleksander M. Grabiec, and Mark A. Travis
2. A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages
Chandrayana Mukherjee, Christine Hale, and Subhankar Mukhopadhyay
3. Growing Murine Bone Marrow-Derived Macrophages
Anaïs Assouvie, Lisa P. Daley-Bauer, and Germain Rousselet
4. Immortalization of Murine Bone Marrow-Derived Macrophages
Dominic De Nardo, Dhan V. Kalvakolanu, and Eicke Latz5. Isolation and Identification of Murine Serous Cavity Macrophages
Calum C. Bain and Stephen J. Jenkins
6. Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies
Shaikh M. Atif, Sophie L. Gibbings, and Claudia V. Jakubzick
7. Isolation and Phenotyping of Adult Mouse Microglial Cells
Kathleen Grabert and Barry W. McColl
8. Isolation and Phenotyping of Bone Marrow Macrophages
Marion Chalot
Vanessa Petit
10. Phenoytyping Tumor Associated Macrophages
R. Ian Cumming and Yen-Rei A. Yu
11. Activating Murine Macrophages In Vitro
Elizabeth Dalby
12. Polarizing Macrophages In Vitro
Xuan Huang, Yong Li, Mingui Fu, and Hong-Bo Xin
13. Viral Replication Assay in Bone Marrow-Derived Macrophages
Linda Roback and Lisa P. Daley-Bauer
14. Macrophage Bactericidal Assays
Mourad Aribi
15. Quantitative Phagocytosis Assays in Primary and Cultured Macrophages
Fernando Montaño, Sergio Grinstein, and Roni Levin
16. Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM)
Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, and Florence Niedergang17. Chromatine Immunoprecipitation in Macrophages
Germain Rousselet
18. Transfecting Macrophages
Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Wittig, and Stefan Lorkowski
19. In Vitro Migration Assays
Lewis Taylor, Carlota Recio, David R. Greaves, and Asif J. Iqbal
20. Cytokine-Induced Acute Inflammatory Monarticular Arthritis
Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, and Andrew D. Cook
Dominik Ruckerl
22. Genetic Models of Macrophage Depletion
Li Hua, Jiayuan Shi, Leonard D. Shultz, and Guangwen Ren
23. Depleting Macrophages In Vivo with Clodronate-Liposomes
Stéphanie G. Moreno
24. Cre Driver Mice Targeting Macrophages
Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, and Guangwen Ren
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts