Metalloproteins, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1876

This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems.

Part I.   Metalloproteins

      1. Nitrogenases

          Nate Sickerman, Yilin Hu, and Markus W. Ribbe

      2. Bacteriochlorophyll and Coenzyme F430

          Jürgen Moser and Gunhild Layer

      3. Carbon Monoxide Dehydrogenases

          Jae-Hun Jeoung, Berta M. Martins, and Holger Dobbek

      4. Molybdenum-Containing Enzymes 

          Dimitri Niks and Russ Hille

      5. Hydrogenases

          Nathaniel S. Sickerman and Yilin Hu

Part II. Genetic and Biochemical Methods

  6. Genomic Manipulations of the Diazotroph Azotobacter vinelandii

      Patricia Dos-Santos

  7. Purification of Nitrogenase Proteins

      Chi Chung Lee, Markus W. Ribbe, and Yilin Hu

  8. Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni²⁺-Sirohydrochlorin a,c-Diamide Reductase

      Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, and Gunhild Layer       

  9. Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor

      Paul Kaufmann, Chantal Iobbi-Nivol, and Silke Leimkühler

Part III.   Structural and Spectroscopic Methods

      10. Crystallization of Nitrogenase Proteins

            Belinda B. Wenke, Renee J. Arias, and Thomas Spatzal

      11. X-Ray Crystallography of Carbon Monoxide Dehydrogenases

      12. X-Ray Absorption Spectroscopy of Metalloproteins

      13. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins

            Andrew Jasniewski, Yilin Hu, and Markus Ribbe

      14. Magnetic Circular Dichroism Spectroscopy of Metalloproteins

            Brian J. Hales

Part IV.   Chemical methods and Theoretical calculations

      15. Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site

            Kazuki Tanifuji and Yasuhiro Ohki

      16. Computational Methods for Modeling Metalloproteins

            Martin Stiebritz and Yilin Hu         

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts