Organoids, 1st ed. 2019
Stem Cells, Structure, and Function
Methods in Molecular Biology Series, Vol. 1576

Coordinator: Turksen Kursad

Language: English

116.04 €

Subject to availability at the publisher.

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363 p. · 17.8x25.4 cm · Hardback
This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.

1. A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment

            Magdalena Sutcliffe and Madeline A. Lancaster

2. Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells

            Eva Tomaskovic-Crook and Jeremy M. Crook

3. Organoid Assay of Human Cancer Stem Cells

            Yohei Shimono, Piero Dalerba, Junko Mukohyama, Taichi Isobe, Darius Johnston, and Akira Suzuki

4. Construction of Thymus Organoids from Decellularized Thymus Scaffolds

            Asako Tajima, Isha Pradhan, Xuehui Geng, Massimo Trucco, and Yong Fan

5. Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres

            Robert E. Hynds, Colin R. Butler, Sam M. Janes, and Adam Giangreco

6. Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells

            Mahboobe Ghaedi and Laura E. Niklason

7. Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix

            Hiroko Hisha and Hiroo Ueno

8. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension

            Valentina Benedetti, Valerio Brizi, and Christodoulos Xinaris

9. Efficient Culture of Intestinal Organoids with Blebbistatin

            Zhen Qi and Ye-Guang Chen

10. Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion

            Dustin J. Flanagan, Renate H.M. Schwab, Bang M. Tran, Toby J. Phesse, and Elizabeth Vincan

11. The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine

            Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, and Maja Grabacka

12. New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice

            Toshio Takahashi

13. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System

            Jessica L. Forbester, Nicholas Hannan, Ludovic Vallier, and Gordon Dougan

14. Murine Colonic Organoid Culture System and Downstream Assay Applications

            Yang-Yi Fan, Laurie A. Davidson, and Robert S. Chapkin

15. Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions

            Giulia Nigro, Melissa Hanson, Cindy Fevre, Marc Lecuit, and Philippe J. Sansonetti

16. The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism

            Diana Li, Hongli Dong, and Alison B. Kohan

17. Oncogenic Transformation of Human-Derived Gastric Organoids

            Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, and Yana Zavros

18. Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation

            Yun Chen, Chuan Li, Sheng-Hong Tseng, and Ya-Hui Tsai

19. Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections

            Winnie Y. Zou, Sarah E. Blutt, Sue E. Crawford, Khalil Ettayebi, Xi-Lei Zeng, Kapil Saxena, Sasirekha Ramani, Umesh Karandikar, Nicholas C. Zachos, and Mary K. Estes

20. Study Bacterial-Host Interactions Using Intestinal Organoids

            Yong-guo Zhang and Jun Sun

21. Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering

            Megan K. Eldred, Leila Muresan, and William A. Harris

22. Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo

            Kai Tong, Mahendra Wagle, and Su Guo

23. Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells

            Yoni Baert, Charlotte Rombaut, and Ellen Goossens

24. Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids

            Tiziana A.L. Brevini, Elena F.M. Manzoni, Sergio Ledda, and Fulvio Gandolfi

25. Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly

            Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, and Sinan Güven

26. Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying

            Mozhdeh Imaninezhad, Era Jain, and Silviya Petrova Zustiak

27. Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues

            Soojung Hahn and Jongman Yoo

28. Drug Sensitivity Assays of Human Cancer Organoid Cultures

            Hayley E. Francies, Andrew Barthorpe, Anne McLaren-Douglas, William J. Barendt, and Mathew J. Garnett

Includes cutting-edge methods and protocols for the study of organoids

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts