Post-Translational Modification of Proteins (3^rd Ed., 3rd ed. 2019)
Tools for Functional Proteomics
Methods in Molecular Biology Series, Vol. 1934

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins.  In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Preface…

Table of Contents…

Contributors…

1. Web-Based Computational Tools for the Prediction and Analysis of Post-Translational Modifications of Proteins

Vladimir A. Ivanisenko, Timofey V. Ivanisenko, Olga V. Saik, Pavel S. Demenkov, Dimitry A. Afinnikov, and Nikolay A. Kolchanov

2. Detection of Post-Translational Modifications by Fluorescent Staining of Two-Dimensional Gels

Archana M. Jacob and Chris W. Turck

3. Analysis of Ser/Thr Linked Sugar Chains

Kalyan R. Anumula

4. Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling

Detlef Grunow and Véronique Blanchard

5. Enzymatic Sequence Analysis of N-Glycans By Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures

Christoph Kannicht, Detlef Grunow, and Lothar Lucka

6. Chromatographic Profiling of N-Glycans

Martin Gohlke and Véronique Blanchard

7. Activity Determination of Glycosyltransferases

Kalyan R. Anumula

8. Characterization of Site-Specific N-Glycosylation

Helga Hevér, Zsuzsanna Darula, and Katalin F. Medzihradszky

9. Glycosylation of Type I Collagen

Mitsuo Yamauchi, Marnisa Sricholpec, Masahiko Terajima, Kenneth B. Tomer, and Irina Perdivara

10. Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa

Nahid Azzouz, Peter Gerold, and Ralph T. Schwarz

11. Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods

Christof Lenz

12. Mass Spectrometry-Based Method for Detection and Identification of Free Thiol Groups in Proteins

Barbara A. Solecka, Christoph Weise, and Christoph Kannicht

Carol E. Parker, Maria R. E. Warren Hines, Viorel Mocanu, Susanna F. Greer, and Christoph H. Borchers

14. Analysis of Sumoylation

Jan Rettich and Andrea Pichler

15. An Approach for the Identification of Proteins Modified by ISG15

Tomoharu Takeuchi, Shun Koinuma, Hideyoshi Yokosawa, and Yoichiro Arata

16. α-Amidated Peptides: Approaches for Analysis

Gregory P. Mueller, Rachel C. Lazarus, and William J. Driscoll

17. S-Acylation of Proteins

Larisa Kordyukova, Ludwig Krabben, Marina Serebryakov, and Michael Veit

18. Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins

Randy J. Arnold, Suraj Saraswat, and James P. Reilly

19. Lysine Hydroxylation and Cross-Linking of Collagen

Mitsuo Yamauchi, Masahiko Terajima, and Masashi Shiiba

Index...