Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.

1. How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp J. Sung, K.I. Mortensen, J.A. Spudich and H. Flyvbjerg 2. Studying the Mechanochemistry of Processive Cytoskeletal Motors with an Optical Trap V. Belyy and A. Yildiz 3. Single-Molecule Optical Trapping Techniques to Study Molecular Mechanisms of a Replisome B. Sun and M.D. Wang 4. Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy M. H. Shabestari, A.E.C. Meijering, W.H. Roos, G.J. L. Wuite and E.J.G. Peterman 5. Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping C.-T. Lin and T. Ha 6. High-Resolution Optical Tweezers Combined with Single-Molecule Confocal Microscopy K.D. Whitley, M.J. Comstock and Y.R. Chemla# 7. Integrating Optical Tweezers, DNA Tightropes and Single Molecule Fluorescence Imaging: Pitfalls and Traps J. Wang, J.T. Barnett1, M.R. Pollard and N.M. Kad 8. Single-Stranded DNA Curtains for Studying Homologous Recombination C.J. Ma, J.B. Steinfeld and E.C. Greene 9. Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of  DNA Mismatch Repair M.W. Brown, A.D.L. Torre and I.J. Finkelstein 10. Single-Molecule Insight into Target Recognition by CRISPR-Cas Complexes M. Rutkauskas, A. Krivoy, M. Szczelkun, C. Rouillon and R. Seidel 11. Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules C. Duboc, J. Fan, E.T. Graves and T.R. Strick 12. Measuring Force Induced Dissociation Kinetics of Protein Complexes Using Single Molecule Atomic Force Microscopy K. Manibog, C.-F. Yen and S. Sivasankar 13. Improved Force Spectroscopy Using Focused-Ion-Beam Modified Cantilevers J.K. Faulk, D.T. Edwards, M.S. Bull and T.T. Perkins 14. Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul and A. Meller 15. Subangstrom Measurements of Enzyme Function using a Biological Nanopore, SPRNT A.H. Laszlo, I.M. Derrrington and J.H. Gundlach 16. Multiplexed, Tethered Particle Microscopy for Studies of  DNA-Enzyme Dynamics S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap and L. Finzi

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods in single-molecule enzymology
• Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion