Sphingosine-1-Phosphate (2^nd Ed., 2nd ed. 2018)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1697

This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field.

1. Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay

            Jonathan K. Fleming and Jonathan M. Wojciak

2. An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity

            Melissa R. Pitman, Lorena T. Davies, and Stuart M. Pitson

3. Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells

4. Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry

            Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, and Andrew J. Morris

5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines

            Gary M. Reynolds, Barbara Visentin, and Roger Sabbadini

            Jun-ichi Morishige, Ryouhei Yamashita, Tamotsu Tanaka, and Kiyoshi Satouchi

7. A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes

            Naoki Kobayashi and Tsuyoshi Nishi

8. Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry

            Jianhong Zhang, Annie Bang, and Stephen J. Lye

9. S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses

            Camille Duran, Roland Kaunas, and Kayla Bayless

10. In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate

11. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor

            Raymond C.B. Wong, Martin F. Pera, and Alice Pébay

12. Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors

            Phillip Callihan, Mohammed Alqinyah, and Shelley B. Hooks

13. Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation

            Guanghu Wang, Stefka D. Spassieva, and Erhard Bieberich

14. 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering

            Shrestha Priyadarsini, Sarah E. Nicholas, and Dimitrios Karamichos

Includes cutting-edge techniques for the study of sphingosine-1-phosphate

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Includes supplementary material: sn.pub/extras