Description
Tau Protein, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1523
Coordinator: Smet-Nocca Caroline
Language: EnglishSubject for Tau Protein:
Publication date: 07-2018
Support: Print on demand
Publication date: 12-2016
Support: Print on demand
Description
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This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology. Divided into five parts that illustrate the underlying molecular mechanisms of Tau functions and dysfunctions in Alzheimer?s disease and other dementia referred to as tauopathies, this volume discusses timely topics such as: conformational studies of native tau protein and investigation of its physiological function in microtubules binding and tubulin polymerization; in vitro methods of formation and detection of tau oligomers and PHF-like fibrils; characterization and in vitro introduction of post-translational modifications on tau proteins for further functional studies; analytical tools for the detection of tau proteins, their modifications and cellular interactions, and MAPT gene mutations in various biological samples; and cellular and in vivo models for the investigations of tau physiopathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Tau Protein: Methods and Protocols is a valuable resource that addresses the most recent aspects of Alzheimer?s disease research related to Tau protein and state-of-the-art experimental techniques. It targets a broad scientific audience including molecular biologists and biochemists, as well as all researchers interested in exploring the functions of tau proteins.
Preface
Table of Contents
Contributors
Part I Native Tau Protein: Conformational Studies and Tubulin Interactions
1. Conformational Dynamics of Intracellular Tau Protein Revealed by CD and SAXS
Nalini Vijay Gorantla, Alexander V. Shkumatov, and Subashchandrabose Chinnathambi
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras