PART I: PREPARING VIRUS AND VIRUS-DERIVED NANOARCHITECTURES AS CONTAINERS, CARRIER SCAFFOLDS, AND STRUCTURE-DIRECTING AGENTS: FROM PARTICLES TO FUNCTIONAL SUPER-ASSEMBLIES
1. The Production in Plants of Mosaic Turnip Crinkle Virus-like Particles Derived by Co-infiltration of Wild-Type and Modified Forms of Virus Coat Protein
Roger Castells-Graells, George P. Lomonossoff and Keith Saunders
2. Isolation and Characterization of Two Distinct Types of Unmodified Spherical Plant Sobemovirus-like Particles for Diagnostic and Technical Uses
Ina Balke, Gunta Reseviča, and Andris Zeltins
3. RNA-directed Assembly of Tobacco Mosaic Virus (TMV)-Like Carriers with Tunable Fractions of Differently Addressable Coat Proteins
Sabine Eiben
4. Fabrication of Tobacco Mosaic Virus-like Nanorods for Peptide Display
Emily J. Larkin, Adam D. Brown, and James N. Culver
5. In planta Production of Fluorescent Filamentous Plant Virus-based Nanoparticles
Sourabh Shukla, Christina Dickmeis, Rainer Fischer, Ulrich Commandeur, and Nicole F. Steinmetz
6. Self-assembling Plant-derived Vaccines against Papillomaviruses
Emanuela Noris
7. Recombinant Expression of Tandem-HBc Virus-like Particles (VLPs)
Sam L Stephen, Lucy Beales, Hadrien Peyret, Amy Roe, Nicola J. Stonehouse, and David J. Rowlands
8. Production and Application of Insect Virus-based VLPs
Radhika Gopal and Anette Schneemann
9. Nanomanufacture of Free-standing, Porous, Janus-Type Films of Polymer-Plant Virus Nanoparticle Arrays
Brylee David B. Tiu, Rigoberto C. Advincula, and Nicole F. Steinmetz
10. Self-assembly of Rod-like Bionanoparticles at Interfaces and in Solution
Ye Tian and Zhongwei Niu
11. Bottom-up Assembly of TMV-based Nucleoprotein Architectures on Solid Supports
Christina Wege and Fabian Eber
PART II: FUNCTIONAL COMPOUNDS IN VIRUS-BASED CONTAINERS: FROM PREPARATION TO APPLICATIONS OF HYBRID NANOOBJECTS
12. Internal Deposition of Cobalt Metal and Iron Oxide within CPMV eVLPs
Alaa A. A. Aljabali and David J. Evans
13. Plant Virus-based Nanoparticles for the Delivery of Agronomic Compounds as a Suspension Concentrate
Richard H. Guenther, Steven A. Lommel, Charles H. Opperman, and Tim L. Sit
14. Nanowires and Nanoparticle Chains inside Tubular Viral Templates
Kun Zhou and Qiangbin Wang
15. In vitro-Reassembled Plant Virus-Like Particles of Hibiscus Chlorotic Ringspot Virus (HCRSV) as Nano-protein Cages for Drugs
Sek-Man Wong and Yupeng Ren
16. CCMV-based Enzymatic Nanoreactors
Mark V. de Ruiter, Rindia M. Putri, and Jeroen J.L.M. Cornelissen
17. Protocol for the Efficient Cell-free Synthesis of Cowpea Chlorotic Mottle Virus-like Particles Containing Heterologous RNAs
Rees F. Garmann, Charles M. Knobler, and William M. Gelbart
18. Packaging DNA Origami into Viral Protein Cages
Veikko Linko, Joona Mikkilä, and Mauri A. Kostiainen
19. In Vitro Assembly of Virus-Derived Designer Shells around Inorganic Nanoparticles
Stella E. Vieweger, Irina B. Tsvetkova, and Bogdan G. Dragnea
20. In Vivo Packaging of Protein Cargo inside of Virus-Like Particle P22
Kimberly McCoy and Trevor Douglas
21. Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids
Ioana L. Aanei, Jeff E. Glasgow, Stacy L. Capehart, and Matthew B. Francis
22. Delivering Cargo: Plant-based Production of Bluetongue Virus core- and Virus-like Particles Containing Fluorescent Proteins
Eva C. Thuenemann and George P. Lomonossoff
PART III: FUNCTIONS EXPOSED ON VIRUS BACKBONES AND COMBINED APPROACHES: FROM FABRICATION TO TECHNICAL USES OF HYBRID PARTICLES, FILMS, AND MATERIALS
23. Bioinspired Silica Mineralization on Viral Templates
Christina Dickmeis, Klara Altintoprak, Patrick van Rijn, Christina Wege, and Ulrich Commandeur
24. Propagation and Isolation of Tobacco Mosaic Virus Which Surface Displays Metal Binding and Reducing Peptides for Generation of Gold Nanoparticles
Andrew J. Love and Michael E. Taliansky
25. TMV-templated Formation of Metal and Polymer Nanotubes
Alexander M. Bittner
26. Semiconducting Hybrid Layer Fabrication Scaffolded by Virus Shells
Petia Atanasova
27. Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits
Christina Wege and Fania Geiger
28. Drug-loaded Plant-virus Based Nanoparticles for Cancer Drug Delivery
Michael A. Bruckman, Anna E. Czapar, and Nicole F. Steinmetz
29. Construction of Artificial Enzymes on a Virus Surface
Chunxi Hou and Junqiu Liu
30. Redox-Immuno Functionalized Potyvirus Nanoparticles for High Resolution Imaging by AFM-SECM Correlative Microscopy
Agnès Anne, Arnaud Chovin, Christophe Demaille, and Thierry Michon
31. Presenting Peptides at the Surface of Potyviruses in planta
Flora Sánchez and Fernando Ponz
32. Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials
Hyo-Eon Jin and Seung-Wuk Lee
33. Displaying Whole-chain Proteins on Hepatitis B virus Capsid-like Particles
Julia Heger-Stevic, Philipp Kolb, Andreas Walker, and Michael Nassal
34. Dual-functionalized Virus-gold Nanoparticles Clusters for Biosensing
Carissa M. Soto and Walter J. Dressick
35. TMV-based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications
Claudia Koch, Arshak Poghossian, Christina Wege, and Michael J. Schöning
36. Integrated Methods to Manufacture Hydrogel Microparticles Containing Viral-metal Nanocomplexes with High Catalytic Activity
Cuixian Yang, Eunae Kang, and Hyunmin Yi
37. Integrated Methods to Manufacture Hydrogel Microparticles with High Protein Conjugation Capacity and Binding Kinetics via Viral Nanotemplate Display
Sukwon Jung, Christina L. Lewis, and Hyunmin Yi
38. Interactions between Plant Viral Nanoparticles (VNPs) and Blood Plasma Proteins, and their Impact on the VNP In Vivo Fates
Andrzej S. Pitek, Frank A. Veliz, Slater A. Jameson, and Nicole F. Steinmetz
39. Fabrication of Plant Virus- based Thin Films to Modulate the Osteogenic Differentiation of Mesenchymal Stem Cells
Kamolrat Metavarayuth, Huong Giang Nguyen, and Qian Wang
40. Dual Surface Modification of Genome-free MS2 Capsids for Delivery Applications
Ioana L. Aanei and Matthew B. Francis
41. Virus-Based Cancer Therapeutics for Targeted Photodynamic Therapy
Binrui Cao, Hong Xu, Mingying Yang, and Chuanbin Mao