Chemokines

Language: English

172,74 €

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Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.

1. Chemokine Detection Using Receptors Immobilized on an Spr Sensor Surface
José Miguel Rodríguez-Frade, Laura Martínez-Muñoz, Ricardo Villares, Graciela Cascio, Pilar Lucas, Rosa P. Gomariz and Mario Mellado
2. Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic  Devices
Jiqing Sai, Matthew Rogers, Kathryn Hockemeyer, John P. Wikswo and Ann  Richmond
3. Generating Chemokine Analogues with Enhanced Pharmacological Properties using Phage Display
Karim Dorgham, Fabrice Cerini, Hubert Gaertner, Astrid Melotti, Irène Rossitto- Borlat, Guy Gorochov and Oliver Hartley
4. Methods for the Recognition of GAG-Bound Chemokines
Pauline Bonvin, Nicolas Fischer and Amanda Proudfoot
5. Monitoring Scavenging Activity of Chemokine Receptors
Barbara Moepps and Marcus Thelen
6. Dual Color Luciferase Complementation for Chemokine Receptor Signaling
Kathryn E. Luker and Gary D. Luker
7. Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer
J. Bonneterre, N. Montpas, C. Boularan, C. Galés and N. Heveker
8. Probing Biased Signaling in Chemokine Receptors
Roxana-Maria Amarandi, Gertrud Malene Hjortø, Mette Marie Rosenkilde and Stefanie Karlshøj
9. Mutagenesis by Phage Display
Pauline Bonvin, Christine Power, Amanda Proudfoot and Steven Dunn
10. Studying Chemokine Control of Neutrophil Migration in Vivo in a Murine Model of Inflammatory Arthritis
Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster
11. Production of Chemokine/Chemokine Receptor Complexes for Structural and Biophysical Studies
Martin Gustavsson, Yi Zheng and Tracy M. Handel
12. In Vivo Models to Study Chemokine Biology
F.A. Amaral, D. Boff and M.M. Teixeira
13. Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
Adriano Marchese 
14. Active Shaping of Chemokine Gradients by Atypical Chemokine Receptors: a 4D live Cell Imaging Migration Assay
Kathrin Werth and Reinhold Förster
15. The Role of Chemokine and Glycosaminoglycan Interaction in Chemokine- Mediated Migration in Vitro and in Vivo
Irene del Molino del Barrio, John Kirby and Simi Ali
16. Examining Roles of Glycans in Chemokine-Mediated Dendritic-Endothelial Cell Interactions
Xin Yin, Scott C. Johns, Roland El Ghazal, Catherina L. Salanga, Tracy M. Handel and Mark M. Fuster
17. Preparation and Analysis of N-terminal Chemokine Receptor Sulfopeptides  Using Tyrosylprotein Sulfotransferase Enzymes
Christoph Seibert, Anthony Sanfiz, Thomas P. Sakmar, and Christopher T. Veldkamp
18. Disulfide Trapping for Modeling and Structure Determination of Receptor:Chemokine Complexes
Irina Kufareva, Martin Gustavsson, Lauren G. Holden, Ling Qin, Yi Zheng and Tracy M. Handel
19. Analysis of G Protein and ß-Arrestin Activation in Chemokine Receptors  Signaling
Alessandro Vacchini, Marta Busnelli, Bice Chini, Massimo Locati and Elena Monica Borroni
20. Flow Cytometry Detection of Chemokine Receptors for the Identification of  Murine Monocyte and Neutrophil Subsets
Ornella Bonavita, Matteo Massara, Achille Anselmo, Paolo Somma, Hilke Brühl, Matthias Mack, Massimo Locati and Raffaella Bonecchi
21. Molecular Pharmacology of Chemokine Receptors
Raymond H. de Wit, Sabrina M. de Munnik, Rob Leurs, Henry F. Vischer and  Martine J. Smit
22. Preparation and Characterization of Glycosaminoglycan Chemokine Co-Receptors
Nikola Kitic, Martha Gschwandtner, Rupert Derler, Tanja Gerlza and Andreas J. Kungl
23. Production of Recombinant Chemokines and Validation of Refolding
Christopher T. Veldkamp, Chad A. Koplinski, Davin R. Jensen, Francis C. Peterson, Kaitlin M. Smits, Brittney L. Smith, Scott K. Johnson, Christina Lettieri, Wallace G.  Buchholz,Joyce C. Solheim and Brian F. Volkman
24. Quantitative Analysis of Dendritic Cell Haptotaxis
Jan Schwarz, Michael Sixt
  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers research methods in chemokines
  • Contains sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokine, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies