DNA Repair Enzymes: Structure, Biophysics, and Mechanism

DNA Repair Enzymes, Part B, Volume 592 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA Repair Enzymes. Topics in this updated volume include MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes, Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes, Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR, the Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair, and Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes.

1. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes Scott D. Gradia, Justin P. Ishida, Miaw-Sheue Tsai, Chris Jeans, John A. Tainer and Jill O. Fuss 2. Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes David M. Rees, Oliver Willhoft, Chia-Liang Lin, Rohan Bythell-Douglas and Dale B. Wigley 3. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR Matthew K. Thompson, Aaron C. Ehlinger and Walter J. Chazin 4. Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair Peter Friedhoff, Laura Manelyte, Luis Giron-Monzon, Ines Winkler, Flora Groothuizen and Titia K. Sixma 5. Expression and Structural Analyses of Human DNA Polymerase ? (POLQ) Andrew W. Malaby, Sara K. Martin, Richard D. Wood and Sylvie Doublié 6. Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes Malgorzata Figiel and Marcin Nowotny 7. DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage Dimitri Y. Chirgadze, David B. Ascher, Tom L. Blundell and Bancinyane L. Sibanda 8. Single-Particle Electron Microscopy Analysis of DNA Repair Complexes Marta Sawicka, Ricardo Aramayo, Rafael Ayala, Robert Glyde and Xiaodong Zhang 9. Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes that Carry Out DNA Repair Sharonda LeBlanc, Hunter Wilkins, Zimeng Li, Parminder Kaur, Hong Wang and Dorothy A. Erie 10. Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time Muwen Kong, Emily C. Beckwitt, Luke Springall, Neil M. Kad and Bennett Van Houten 11. Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination Michael M. Soniat, Logan R. Myler, Jeffrey M. Schaub, Yoori Kim, Ignacio F. Gallardo and Ilya J. Finkelstein 12. Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography Nadine Samara, Yang Gao, Jinjun Wu and Wei Yang 13. Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases Kyle T. Powers and M.T. Washington 14. Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair Christopher T. Coey and Alexander C. Drohat 15. Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping Jenna M. Hendershot and Patrick J. O’Brien 16. What Combined Measurements from Structures and Imaging Tell Us About DNA Damage Responses Chris A. Brosey, Zamal Ahmed, Susan P. Lees-Miller and John A. Tainer

Scientists interested in modern nucleic acid biology, as well as to scientists outside the field interested in learning how the field has expanded to encompass a broad range of topics, pathways, and methods

• Includes contributions from leading authorities working in enzymology
• Focuses on DNA repair enzymes
• Informs and updates on all the latest developments in the field of enzymology