Description
Human Monoclonal Antibodies (2nd Ed., 2nd ed. 2019)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1904
Coordinator: Steinitz Michael
Language: EnglishSubject for Human Monoclonal Antibodies:
Keywords
transgenic mice; phage display; Alzheimer’s disease; melanoma; T-cells; antibodies
Publication date: 12-2018
483 p. · 17.8x25.4 cm · Hardback
483 p. · 17.8x25.4 cm · Hardback
Description
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This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.
Dedication…
Preface…
Table of Contents…
Contributing Authors…
1. Human Monocolonal Antibodies: The Benefits of Humanization
Herman Waldmann
2. Cancer Immunotherapy: The Dawn of Antibody Cocktails
Ilaria Marrocco, Donatella Romaniello, and Yosef Yarden
3. IgM Natural Autoantibodies in Physiology and the Treatment of Disease
Mahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, and Moses Rodriguez
4. Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies
Madhuri Bhandaru and Anand Rotte
5. An Efficient Method to generate Monoclonal Antibodies from Human B Cells
Jenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, and Patrick C. Wilson
6. Isolation of Antigen-Specific, antibody-Secreting Cells using a Chip-Based Immunospot Array
Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, and Atsushi Muraguchi
7. Purification of Human Monoclonal antibodies and Their Fragments
Nicole Ulmer, Sebastian Vigg, Thomas Müller-Späth, and Massimo Morbidelli
8. One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood
Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, and Benjamin Frey
9. Humanization and Simultaneous Optimization of Monoclonal Antibody
T. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori
10. Antibody Fragments Humanization: Beginning with the End in Mind
Nicolas Aubrey and Philippe Billiald
11. Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice
Susana Magadán Mompó and África González-Fernández
12. Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework
Jeremy D. King and John C. Williams
13. Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors
Keichiro Mihara, Tetsumi Yoshida, and Joyeeta Bhattacharyya
14. Rapid Chimerization of Antibodies
Koji Hashimoto, Kohei Kurosawa, Hidetaka Seo, and Kunihiro Ohta
15. Phage Display Technology for Human Monoclonal Antibodies
Dal Ferro Marco, Rizzo Serena, Rizzo Emanuela, Marano Francesca, Luisis Immacoloata, Tarasiuk Olga, and Daniele Sblattero
16. Recombinant Antibody Selections by Combining Phage and Yeast Display
Fortunato Ferrara, Maria Felicia Soluri, and Daniele Sblattero
17. Epitope Mapping via Phage Display from Single Gene Libraries
Viola Fühner, Philip Alexander Heine, Kilian Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira, Michael Hust, and Giulio Russo
18. A High-Throughput Magnetic Nanoparticle Based Semi-Automated Antibody Phage Display Biopanning
Angela Chiew Wen Ch’ng, Azimah Ahmad, Zoltán Konthur, and Theam Soon Lim
19. A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin
Ivan Babic, Santosh Kesari, and Mark C. Glossy
20. A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors
Patricia Blundell and Richard Pleass
21. A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal Antibodies
Michihiko Aoyama, Minoru Tada, and Akiko Ishii-Watabe
22. “BIClonals”: Production of Bispecific Antibodies in IgG Format in Transiently Transfected Mammalian Cells
Dana Litvak-Greenfeld, Lilach Vaks, Stav Dror, Limor Nahary, and Itai Benhar
23. Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently-Transfected Mammalian Cells
Racheli Birnboim-Perach, Yehudit Grinberg, Lilach Vaks, Limor Nahary, and Itai Benhar
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
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