Immunoproteomics (2^nd Ed., 2nd ed. 2019)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2024

This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field. 

Preface…

Table of Contents…

Contributing Authors…

1. Introduction to the Immune System

Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, and Felicity Stark

Kelly M. Fulton, Isabel Baltat, and Susan M. Twine

3. Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification

Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, and Susan M. Twine

Greg Harris and Wangxue Chen

5. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

Timothy D. Veenstra

6. Enriching for Low Abundance Serum Proteins using ProteoMiner™ and Protein Level HPLC

Sophie Moggridge, Kelly M. Fulton, and Susan M. Twine

7. Identification of the Antigen Content of Electroimmunoprecipitates

N. Helena Beyer and Niels H. H. Heegaard

8. Detection of Human Norovirus-Specific Antibodies using the Luciferase Immunoprecipitation System (LIPS) Assay

Christine M. Tin and Stanislav V. Sosnovtsev

9. Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum

Christie E. Delaney, John F. Kelly, Wen Ding, and Arsalan S. Haqqani

10. Antigen Identification for Cell-Binding Antibodies using Ligand Directed Crosslinking and Biotin Transfer

Tammy-Lynn Tremblay and Jennifer J. Hill

11. Whole Genome-Phage Display Libraries: A Powerful Tool for Antigen Discovery

Elisa Beghetto and Nicola Gargano

Johannes W. Pedersen, Alexander Nøstdal, and Hans H. Wandall

13. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

Fei Wen, Mason R. Smith, and Huimin Zhao

14. Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification

Sarah A. Penny and Stacy A. Malaker

15. Enhanced Mass Spectrometry Detection of MHC Peptides

Rui Chen and Jianjun Li

16. Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles

Rui Chen and Jianjun Li

17. Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways

Stacy A. Malaker and Michael J. Ferracane

18. Genome Based Bioinformatic Prediction of Major Histocompatibility (MHC)

Simon J. Foote

19. Enhancing Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted Database Search Approach

Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, and Shashi Gujar

20. Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics

Arsalan S. Haqqani and Danica B. Stanimirovic

21. Genomics Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases 

Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos,  Volker Stadler, and David K. Han

22. Identification of Antigens in Immune Complexes

Nozomi Aibara and Kaname Ohyama

23. Analysis and Characterization of Immune Cells and their Activation Status by Whole-Cell MALDI TOF Mass Spectrometry

Richard Ouedraogo, Julien Textoris, Laurent Gorvel, Aurélie Daumas, Christian Capo, and Jean-Louis Mege

24. A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza

Kevin M. Downard

25. Immunoproteomic Biomarkers: From Publication to Personalized Medicine

Devanand Pinto