Methionine Dependence of Cancer and Aging, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1866

This book explores the methionine dependence of cancer and its effects on aging, a great story of science that is not widely known. The chapters in this book describe the discovery of methionine dependence of cancer; the molecular basis for the increased methionine demand of cancer cells and tumors; the clinical application of methionine dependence of cancer in PET imaging with [11C]methionine; the development of methioninases as cancer drugs; the anti-aging and anti-diabetes effects of methionine restriction; and the future of targeting methionine in the body for the elimination of cancer and for the extension of a healthy life-span. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Methionine Dependence of Cancer and Aging: Methods and Protocols is an important resource for researchers and scientists wishing to pursue this exciting and vital area of study."

Dedication/Frontis…

Preface/Foreward…

Table of Contents…

Contributing Authors…

1) Methods to Study the Role of Methionine Restricted Diet and Methioninase in Cancer Growth Control

Shushma Chaturvedi and Joeseph R. Bertino

2) Altered Methionine Metabolism in Cancer Cells

Robert M. Hoffman, Peter H. Stern, Dennis W. Coalson, C. Douglas Wallace, and Richard W. Erbe

3) Linkage of Methionine-Dependence and Other Features of Malignancy

Robert M. Hoffman and Richard W. Erbe

4) Isolation and Characterization of Methionine Independent Clones from Methionine Dependent Cancer Cells

Stacey L. Borrego, Da-Wei Lin, and Peter Kaiser

5) Tumor-Specific S/G2-Phase Cell Cycle Arrest of Cancer Cells by Methionine Restriction

Robert M. Hoffman and Shuya Yano  

6) Preclinical Breast Cancer Models to Investigate Metabolic Priming by Methionine Restriction 

Elena Strekalova, Dmitry Malin, Harisha Rajanala, and Vincent L. Cryns

7) Efficacy of Methionine-Restricted Diets on Cancers In Vivo

Robert M. Hoffman, Yasunori Hoshiya, and Weiyan Guo

8) Dietary Methionine-Restriction Based Cancer Chemotherapy in Rodents 

Robert M. Hoffman and Peter H. Stern

9) Clinical Studies of Methionine-Restricted Diets for Cancer Patients

Robert M. Hoffman

Robert M. Hoffman, Yuying Tan, Shukuan Li, Qinghong Han, Shigeo Yagi, Tomoaki Takakura, Akio Takimoto, Kenji Inagaki, and Daizou Kudou

11) Methioninase Cell-Cycle Trap Cancer Chemotherapy

Robert M. Hoffman, Shuya Yano, and Kentaro Igarashi

12) High Efficacy of Recombinant Methioninase on Patient-Derived Orthotopic Xenograft (PDOX) Mouse Models of Cancer

Robert M. Hoffman, Takashi Murakami, Kei Kawaguchi, Kentaro Igarashi, Yuying Tan, Shukuan Li, and Qinghong Han, 

13) Total Methionine Restriction Treatment of Cancer

Robert M. Hoffman, Demetrius M. Kokkinakis, and Eugene P. Frenkel

14) Methioninase gene Therapy

Robert M. Hoffman, Kenji Miki, Waddah Al-Refaie, Mingxu Xu, and Yuying Tan

15) Selenomethionine and Methioninase: Selenium Free Radical Anti-Cancer Activity

Julian E. Spallholz

Robert M. Hoffman, Zhijian Yang, Yuying Tan, Qinghong Han, Shukuan Li, and Shigeo Yagi

17) Pilot Phase 1 Clinical Trial of Methioninase on High-Stage Cancer Patients: Rapid Depletion of Circulating Methionine

Robert M. Hoffman, Yuying Tan, Shukuan Li, Qinghong Han, Jorge Zavala Sr., and Jorge Zavala Jr.  

18) Methionine Gamma Lyase from Clostridium sporogenes Increases the Anti-Cancer Effect of Doxorubicin in A549 Cancer Cells In Vitro and Human Cancer Xenografts

V.S. Pokrovsky, N. Yu Anisimova, D. Zh. Davydov, S.V. Bazhenov, N.V. Bulushova, G.B. Zavilgelsky, V.Y. Kotova, and I.V. Manukhov

19) Methionine Restriction and Life-Span Extension

20) L-[methyl-11C] Methionine-Positron Emission Tomography (MET PET)

Robert M. Hoffman.  

21) Is the Hoffman Effect for Methionine Overuse Analogous to the Warburg Effect for Glucose Overuse in Cancer?

Robert M. Hoffman

22) Recombinant Methioninase as a DNA Demethylation Agent

Robert M. Hoffman and David Machover

23) Homocysteine, Thioretinaco Ozonide and Oxidative Phosphorylation in Cancer and Aging: A Proposed Clinical Trial Protocol

Kilmer S. McCully 

24) Afterword - Oral Methioninase: Answer to Cancer and Foundation of Youth?

Robert M. Hoffman, Qinghong Han, Kei Kawaguchi, Shukuan Li, and Yuying Tan

Epilogue… 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts