Peptide Antibodies, Softcover reprint of the original 1st ed. 2015
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1348

This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.

1. Peptide Antibodies: Past, Present, and Future

            Gunnar Houen

2. The Structure of Natural and Recombinant Antibodies

            Hui Ma and Richard O’Kennedy

3. Prediction of Antigenic B and T Cell Epitopes Via Energy Decomposition Analysis: Description of the Web-Based Prediction Tool BEPPE

            Claudio Peri, Oscar C. Solé, Dario Corrada, Alessandro Gori, Xavier Daura, and Giorgio Colombo

4. Prediction of Antibody Epitopes

            Morten Nielsen and Paolo Marcatili

5. Fmoc Solid-Phase Peptide Synthesis

            Paul Robert Hansen and Alberto Oddo

6. Peptide-Carrier Conjugation

            Paul Robert Hansen

7. Solid Phase Peptide-Carrier Conjugation

            Gunnar Houen and Dorthe T. Olsen

8. Analysis of Peptides and Conjugates by Amino Acid Analysis

            Peter Højrup

9. Characterization of Synthetic Peptides by Mass Spectrometry

            Bala K. Prabhala, Osman Mirza, Peter Højrup, and Paul Robert Hansen

10. Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis

            Karin Hjernø and Peter Højrup

11. Polyclonal Peptide Antisera

            Tina H. Pihl, Kristin E. Engelhart, and Gunnar Houen

12. Production and Screening of Monoclonal Peptide Antibodies

            Nicole Hartwig Trier, Anne Mortensen, Annette Schiolborg, and Tina Friis

13. Production of Epitope-Specific Antibodies by Immunization with Synthetic Epitope Peptide Formulated with CpG-DNA-Liposome Complex Without Carriers

            Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon

14. Thioredoxin-Displayed Multipeptide Immunogens

            Angelo Bolchi, Elena Canali, Andrea Santoni, Gloria Spagnoli, Daniele Viarisio, Rosita Accardi, Massimo Tommasino, Martin Müller, and Simone Ottonello

15. The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies

            Hui Ma and Richard O’Kennedy

16. Isolation of Camelid Single-Domain Antibodies Against Native Proteins Using Recombinant Multivalent Peptide Ligands

            Norah A. Alturki, Kevin A. Henry, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi

17. Generation of TCR-Like Antibodies Using Phage Display

            Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung

18. Structural Characterization of Peptide Antibodies

            Anna Chailyan and Paolo Marcatili

19. Automated High Throughput Mapping of Linear B Cell Epitopes Using a Statistical Analysis of High Density Peptide Microarray Data

            Thomas Østerbye and Søren Buus

20. Characterization of Peptide Antibodies by Epitope Mapping Using Resin-Bound and Soluble Peptides

            Nicole Hartwig Trier

21. Screening and Characterization of Linear B-Cell Epitopes by Biotinylated Peptide Libraries

            Ida Rosenkrands and Anja Olsen

22. Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies

            Angela Filomena, Yvonne Heubach, Markus F. Templin, Thomas O. Joos, Nicole Schneiderhan-Marra, and Oliver Poetz

23. Surface Plasmon Resonance Method to Evaluate Anti-Citrullinated Protein/Peptide Antibody Affinity to Citrullinated Peptides

            Feliciana Real-Fernández, Giada Rossi, Filomena Panza, Federico Pratesi, Paola Migliorini, and Paolo Rovero

24. Specificity Analysis of Histone Modification Specific Antibodies or Reading Domains on Histone Peptide Arrays

            Goran Kungulovski, Ina Kycia, Rebekka Mauser, and Albert Jeltsch

25. Prion-Specific Antibodies Produced in Wild-Type Mice

            Peter M.H. Heegaard, Ann-Louise Bergström, Heidi Gertz Andersen, and Henriette Cordes

26. Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide, and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane

            Takenori Yamamoto, Taisuke Matsuo, Atsushi Yamamoto, Ryohei Yamagoshi,Kazuto Ohkura,Masatoshi Kataoka, and Yasuo Shinohara

27. Immunocytochemical and Immunohistochemical Staining with Peptide Antibodies

            Tina Friis, Klaus Boberg Pedersen, David Hougaard, and Gunnar Houen

28. Designing B-Cell Epitopes for Immunotherapy and Subunit Vaccines

            Harinder Singh, Sudheer Gupta, Ankur Gautam, and Gajendra P.S. Raghava

29. Enterovirus-Specific Anti-Peptide Antibodies

            Chit Laa Poh, Katherine Kirk, Hui Na Chua, and Lara Grollo

30. Therapeutic HIV Peptide Vaccine

            Anders Fomsgaard

Includes cutting-edge methods and protocols for studying peptide antibodies

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts