Traumatic and Ischemic Injury, Softcover reprint of the original 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1717

This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumatic and ischemic injury. The chapters in this book discuss topics, such as animal models and techniques commonly used to study traumatic brain injury, hemorrhagic shock, ischemic and hemorrhagic strokes, sepsis, burn injury, hind limb ischemia, myocardial-ischemia-reperfusion injury, intracranial pressure, global hypoxia-induced perinatal seizures models, and in vitro models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Traumatic and Ischemic Injury: Methods and Protocols is a valuable resource for novices with limited experience to help them initiate new research projects, and established researchers to help them identify comparable approaches and strategies to their studies in this field.

Preface

Table of Contents

Contributing Authors

1. Mouse Injury Model of Polytrauma and Shock

Juan C. Mira, Dina C. Nacionales, Tyler J. Loftus, Ricardo Ungaro, Brittany Mathias, Alicia M. Mohr, Lyle L. Moldawer, and Philip A. Efron

2. Measurement of Intracranial Pressure in Freely Moving Rats

Michael R. Williamson, Roseleen F. John, and Frederick Colbourne

3. A Lateral Fluid Percussion Injury Model for Studying Traumatic Brain Injury in Rats

Paige S. Katz and Patricia E. Molina

4. A Mouse Controlled Cortical Impact Model of Traumatic Brain Injury for Studying Blood-Brain Barrier Dysfunctions

Himakarnika Alluri, Chinchusha Anasooya Shaji, Matthew L. Davis, and Binu Tharakan

5. A Rat Model of Hemorrhagic Shock for Studying Vascular Hyperpermeability

Prince Esiobu and Ed W. Childs

6. Assessment of Cardiovascular Function and Microvascular Permeability in a Conscious Rat Model of Alcohol Intoxication Combined With Hemorrhagic Shock and Resuscitation

Travis M. Doggett, Jared J. Tur, Natascha G. Alves, Sarah Y. Yuan, Srinivas M. Tipparaju, and Jerome W. Breslin

7. Intracerebral Hemorrhage in Mice

8. A Rat Burn Injury Model for Studying Changes in Microvascular Permeability

Katie Wiggins-Dohlvik and Binu Tharakan

9. Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

Mary Susan Lopez and Raghu Vemuganti

10. A Complete Guide to Using the Endothelin-1 Model of Stroke in Conscious Rats for Acute and Long Term Recovery Studies

Hima C.S. Abeysinghe and Carli L. Roulston

11. A Murine Model of Hind Limb Ischemia to Study Angiogenesis and Arteriogenesis

Jun Yu and Alan Dardik

12. A Murine Model of Myocardial Ischemia-Reperfusion Injury

Zhaobin Xu, Kevin E. McElhanon, Eric X. Beck, and Noah Weisleder

13. A Rat Model of Perinatal Seizures Provoked by Global Hypoxia

Jason A. Justice and Russell M. Sanchez

14. Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice

Santosh Kumar Mishra and Soumen Choudhury

15. Methods to Study the Innate Immune Response to Sepsis

Wendy E. Walker

16. An Ovine Model for Studying the Pathophysiology of Septic Acute Kidney Injury

Yugeesh R. Lankadeva, Junko Kosaka, Roger G. Evans, and Clive N. May

17. An In Vitro Model of Traumatic Brain Injury

Ellaine Salvador, Malgorzata Burek, and Carola Y. Förster

18. An In Vitro Oxygen-Glucose Deprivation Model for Studying Ischemia-Reperfusion Injury of Neuronal cells

Myoung-gwi Ryou and Robert T. Mallet

19. Measurement of Microvascular Endothelial Barrier Dysfunction and Hyperpermeability In Vitro

Bobby Darnell Robinson, Chinchusha Anasooya Shaji, Angela Lomas, and Binu Tharakan