Angiogenesis Protocols (3^rd Ed., 3rd ed. 2016)
Methods in Molecular Biology Series, Vol. 1430

In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols, Third Edition also guides readers through new protocols on chorioallantoic membrane models, corneal pocket assays to assess angio- and lymphangiogenesis, models of muscle angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and senescence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Angiogenesis Protocols, Third Edition provides not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Part I: Microscopic Assessment

1.         Assessing Tumor Angiogenesis in Histological Samples

Jia-Min Pang, Nicholas Jene, and Stephen B Fox

2.         Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis

Daniel J. Royston, Steven Clasper, and David G. Jackson

Narmeen S. Ahmad, Stewart G. Martin, and Sarah J. Storr

Part II: Cell Isolation Techniques

4.         Isolation and Culture of Human Endothelial Cells from Micro- And Macro-Vessels

Peter W Hewett

5.         Isolation, Identification and Culture of Human Lymphatic Endothelial Cells

Zerina Lokmic

Jessal J Patel and Richard CM Siow

7.         Isolation and Transfection of Primary Culture Bovine Retinal Pericytes

Part III: In Vitro Techniques

8.         In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation And Matrix Proteolysis

Suzanne A Eccles, William Court, and Lisa Patterson

9.         Tube Forming Assays

Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles, and Roy Bicknell

10.       In Vitro Co-Culture Assays of Angiogenesis

Mark Richards and Harry Mellor

Larissa Pfisterer and Thomas Korff

12.       Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study Of Angiogenesis

Fatemeh Sharifpanah and Heinrich Sauer

13.       Human Arterial Ring Angiogenesis Assay

Giorgio Seano and Luca Primo

Nina Zippel, Yindi Ding, and Ingrid Fleming

15.       Quantitative Imaging-Based Examination of Pericyte Control of Endothelial Growth Dynamics and Angiogenesis.

16.       Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature

Lynn M. Butler, Helen M. Mcgettrick, and Gerard B. Nash

Part IV: In Vivo Techniques

17.       Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy

18.       Quantitative Estimation of Tissue Blood Flow Rate

Gillian M Tozer, Vivien E Prise, and Vincent J Cunningham

Elena I. Deryugina

20.       The Rabbit Corneal Pocket Assay

Lucia Morbidelli and Marina Ziche

21.       The Corneal Micropocket Assay:  A Model of Angiogenesis and Lymphangiogenesis

Shintaro Nakao and Ali Hafezi-Moghadam

22.       Models of Oxygen Induced Retinopathy in Rodents

Melissa V. Gammons and David O. Bates

23.       The Sponge Implant Model of Angiogenesis

Silvia Passos Andrade and Mônica Alves Neves Diniz Ferreira

24.       Measurement of Angiogenesis, Arteriolargenesis and Lymphangiogenesis Phentotypes by Use of Two-Dimensional Mesenteric Angiogenesis Assay

Andrew V. Benest and David O. Bates

25.       In Vivo Models of Muscle Angiogenesis

Stuart Egginton

26.       Use of the Hollow Fibre Assay to Evaluate Agents that Target the Tumor Neovasculature

Patricia A. Cooper and Steven D. Shnyder

27.       Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos

Emma Ristori, Sandra Donnini, and Marina Ziche

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras