Cardiovascular development, Softcover reprint of the original 1st ed. 2012
Methods and Protocols

Methods in Molecular Biology Series, Vol. 843

Coordinators: Peng Xu, Antonyak Marc

Language: Anglais

Approximative price 152.96 €

In Print (Delivery period: 15 days).

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Cardiovascular Development
Publication date:
Support: Imprimé à la demande

Approximative price 152.96 €

In Print (Delivery period: 15 days).

Add to cartAdd to cart
Cardiovascular development
Publication date:
· 17.8x25.4 cm · Paperback
sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Preface

Contributors

 

Part I: Model organisms

1.       Use of Whole Embryo Culture for Studying Heart Development

            Calvin T. Hang, and Ching-Pin Chang

 

2.       Quantifying Cardiac Functions in Embryonic and Adult Zebrafish

Tiffany Hoage, Yonghe Ding, and Xiaolei Xu

 

3.       Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting

            Ching-Pin Chang

 

4.       Morpholino Injection in Xenopus

            Panna Tandon, Chris Showell,  Kathleen Christine, and Frank L Conlon

 

5.       Chicken Chorioallantoic Membrane Angiogenesis Model

Domenico Ribatti

 

6.       Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography

            Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin

 

7.       Whole-mount Confocal Microscopy for Vascular Branching Morphogenesis

Yoh-suke Mukouyama, Jennifer James, Joseph Nam, and Yutaka Uchida

 

8.       Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining

             Yang Liu, Marc Antoniyak, and Xu Peng

           

9.       Miniaturized Assays of Angiogenesis in Vitro

             May J. Reed and Robert B. Vernon

 

Part II: Cell and Molecular Biology Methods

10.   Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay

            Ching-Pin Chang, Yiqin Xiong, and Bin Zhou.

 

11.   Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility

 

            Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor

 

12.   Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining

 

            Yanping Zhang and L. Bruno Ruest

 

13.   Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte

 

Jieli Li, Marc Antonyak, and Xu Peng

 

14.   Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

 

             Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng

 

15.   Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells

 

Bin Zhou and William T. Pu

 

16.   Vascular Smooth Muscle Cells:  Isolation, Culture, and Characterization

 

            Jan Patterson, Rick Metz, and Emily Wilson

 

17.   C-kit Expression Identifies Cardiac Precursor Cells

 

            Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

 

18.   Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols

 

            Dongfei Qi and Mingui Fu

 

19.   Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

 

            Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

 

20.   Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,   

            Xu Peng, and David E. Dostal

 

Part III: New Techniques

21.   The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

 

            Shenyuan Zhang and Hongying Zheng

 

22.   Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

 

            Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 

 

23.   In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

 

            Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

 

24.   Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

 

            Eric A. Bridenbaugh

 

25.   Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

            Vincent VanBuren

 

26.   High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

 

27.   Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

 

            Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor

 

12.   Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining

 

            Yanping Zhang and L. Bruno Ruest

 

13.   Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte

 

Jieli Li, Marc Antonyak, and Xu Peng

 

14.   Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

 

             Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng

 

15.   Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells

 

Bin Zhou and William T. Pu

 

16.   Vascular Smooth Muscle Cells:  Isolation, Culture, and Characterization

 

            Jan Patterson, Rick Metz, and Emily Wilson

 

17.   C-kit Expression Identifies Cardiac Precursor Cells

 

            Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

 

18.   Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols

 

            Dongfei Qi and Mingui Fu

 

19.   Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

 

            Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

 

20.   Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,   

            Xu Peng, and David E. Dostal

 

Part III: New Techniques

21.   The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

 

            Shenyuan Zhang and Hongying Zheng

 

22.   Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

 

            Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 

 

23.   In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

 

            Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

 

24.   Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

 

            Eric A. Bridenbaugh

 

25.   Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

            Vincent VanBuren

 

26.   High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

 

27.   Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

 

Bin Zhou and William T. Pu

 

16.   Vascular Smooth Muscle Cells:  Isolation, Culture, and Characterization

 

            Jan Patterson, Rick Metz, and Emily Wilson

 

17.   C-kit Expression Identifies Cardiac Precursor Cells

 

            Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

 

18.   Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols

 

            Dongfei Qi and Mingui Fu

 

19.   Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

 

            Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

 

20.   Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,   

            Xu Peng, and David E. Dostal

 

Part III: New Techniques

21.   The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

 

            Shenyuan Zhang and Hongying Zheng

 

22.   Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

 

            Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 

 

23.   In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

 

            Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

 

24.   Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

 

            Eric A. Bridenbaugh

 

25.   Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

            Vincent VanBuren

 

26.   High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

 

27.   Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

 

            Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 

 

23.   In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

 

            Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

 

24.   Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

 

            Eric A. Bridenbaugh

 

25.   Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

            Vincent VanBuren

 

26.   High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

 

27.   Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

 

            Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

 

24.   Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

 

            Eric A. Bridenbaugh

 

25.   Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

            Vincent VanBuren

 

26.   High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

 

27.   Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

Michael P. Craig and Jay R. Hove

 

27.   Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

David G. Taylor and Anupama Natarajan

Covers state-of-the-art techniques in the field of cardiovascular development

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts