Clinical Embryology, 2013
A Practical Guide

This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Clinical Embryology covers a variety of topics, including:

- Sperm processing methods

- Embryo culture methods

- Insemination procedures

- Embryo evaluation

- Genetic testing of embryos

- Cryopreservation

- Embryo transfer

Also included are two new chapters on predicting embryo developmental potential and viability using automated time-lapse analysis (Eeva) and predicting embryo implantation potential using Embryoscope.?

Practical for both clinicians and researchers alike, Clinical Embryology brings together all of the need-to-know information about these important topics in reproductive medicine.

Journey of Human Gametes In Vitro: 1978 to 2010.- Part I: Sperm Processing Methods.- Sperm Processing for IVF.- PESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- Part II: Embryo Culture Methods.- Culture Media in IVF: Decisions for the Laboratory.- Short Culture: Day 1/Day 2/Day 3 Embryo Culture.- Extended Culture in IVF.- In Vitro Maturation of Human Oocytes.- In Vivo Embryo Culture Device.- Microfluidics for Gamete Manipulation and Embryo Culture.- Part III: Insemination Procedures.- Intrauterine Insemination.- Conventional IVF Insemination.- Oocyte Denuding.- Intracytoplasmic Sperm Injection.- Part IV: Embryo Evaluation and Grading.- Pronuclear Scoring in Human In Vitro Fertilization.- Cumulative Morphological Assessment of Embryo Quality.- Metablomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability.- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability.- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Real-Time Embryo Monitoring Devices for Embryo Selection.- Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis (Eeva Test).- Predicting Embryo Implantation Potential Using Video Monitoring by the Embryoscope™ Time-Lapse System.- Part V: Genetic Testing of Embryos.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosomal Abnormalities and Gene Defects.- Part VI: Cryopreservation.- Sperm Cryopreservation.- Slow Freezing of Oocytes.- Vitrification: Research in Animal Models.- Oocyte Vitrification.- Slow Freezing of Embryos.- Vitrification of Embryos.- Directional Freezing of Reproduction Cells, Tissues, and Organs.- Ovarian Tissue Cryopreservation.- Part VII: Embryo Transfer.- Assessment of Uterine Receptivity.- Single Embryo Transfer.- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation.

Zsolt Peter Nagy, MD, PhD, HCLD (ABB), EMB (ACE), Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, GA, USA

Alex C. Varghese, PhD, Senior Embryologist, Montreal Reproductive Centre, Montreal, QC, Canada

Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA

Focused, practical guide to clinical embryology

Portable and affordable

State-of-the-art procedures and methods

Includes supplementary material: sn.pub/extras