DNA Repair Enzymes: Cell, Molecular, and Chemical Biology

DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.

1. Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells Nathaniel E. Wiest and Alan E. Tomkinson 2. Proteomic Analyses of the Eukaryotic Replication Machinery David Cortez 3. DNA Fiber Analysis: Mind the Gap! Annabel Quinet, Denisse Carvajal-Maldonado, Delphine Lemacon and Alessandro Vindigni 4. Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells Manas Mondal and Jia Guo 5. Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner Janapriya Saha, Shih-Ya Wang and Anthony J. Davis 6. Base Excision Repair Variants in Cancer Carolyn G. Marsden, Julie A. Dragon, Susan S. Wallace and Joann B. Sweasy 7. Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli Melissa Standley, Jennifer Allen, Layla Cervantes, Joshua Lilly and Manel Camps 8. Sequencing DNA for the Oxidatively Modified Base 8-Oxo-7,8-Dihydroguanine Aaron M. Fleming, Yun Ding and Cynthia J. Burrows 9. Xenopus laevis as Model System to Study DNA Damage Response and Replication Fork Stability Vincenzo Sannino, Federica Pezzimenti, Stefania Bertora and Vincenzo Costanzo 10. Ensemble and Single-Molecule Analysis of NHEJ in Frog Egg Extracts Thomas Graham, Johannes C. Walter and Joseph J. Loparo 11. Analysis of Structure-Selective Endonuclease Activities From Yeast and Human Extracts Joao Matos and Stephen C. West 12. Strategies and Methods of Transcription-Coupled Repair Studies In Vitro and In Vivo  Vitaly Epshtein, Venu Kamarthapu and Evgeny Nudler 13. Reconstituted System for the Examination of Repair DNA Synthesis in Homologous Recombination Youngho Kwon, James M. Daley and Patrick Sung 14. Current and Emerging Assays for Studying the Primer Synthesis Activities of DNA Primases Thomas A. Guilliam and Aidan J. Doherty 15. Electrical Probes of DNA-Binding Proteins Jacqueline K. Barton, Phillip L. Bartels, Yingxin Deng and Elizabeth O’Brien 16. Preparation of Stable Nitrogen Mustard DNA Interstrand Cross-Link Analogs for Biochemical and Cell Biological Studies Alejandra Castaño, Upasana Roy and Orlando D. Schärer

Scientists interested in modern nucleic acid biology, as well as to scientists outside the field interested in learning how the field has expanded to encompass a broad range of topics, pathways, and methods

• Includes contributions from leading authorities working in enzymology
• Focuses on DNA repair enzymes
• Informs and updates on all the latest developments in the field of enzymology