G Protein Coupled Receptors
Modeling, Activation, Interactions and Virtual Screening

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers G protein coupled receptors, and includes chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation.

GPCR Modeling

1. The Protein Local Optimization Program and G-Protein Coupled Receptors: Loop Restoration and Applications to Homology Modeling
2. Modeling Active GPCR Conformations
3. Molecular Modeling of Adenosine Receptors
4. Predicting G Protein-Coupled Receptors Families using Different Physiochemical Properties and Pseudo Amino Acid Composition

Computational Design and Experimental Characterization of GPCR Segment Models

GPCR Interactions with Other Molecules and Cross-Talk

5. G Protein-Coupled Receptor Interaction with Small GTPases
6. Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G Protein Coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA)
7. NMR Methods for Detection of Small Molecule Binding to RGS4
8. Group II Activators of G-protein Signaling: Monitoring the Interaction of Ga with the G-Protein Regulatory Motif in the Intact Cell
9. Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors
10. cAMP Biosensors Applied in Molecular Pharmacological Studies of G Protein-Coupled Receptors
11. Rhodopsin-Lipid Interactions Studied by NMR

GPCR Bias

12. Biasing the Parathyroid Hormone Receptor: Relating In Vitro Ligand Efficacy to In Vivo Biological Activity

Virtual Screening

13. Application of Monte Carlo-based Receptor-Ensemble Docking to Virtual Screening for GPCR Ligands
14. From Heptahelical Bundle to Hits from the Haystack: Structure-Based Virtual Screening for GPCR Ligands

GPCR Activation

15. Kinetics and Dynamics in the G-Protein Coupled Receptor Signaling Cascade
16. Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of G Protein-Coupled Receptors
17. Post-Translation Modification of G Protein-Coupled Receptor in Relationship to Biased Agonism
18. Protein S-Nitrosylation Measurement

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers G protein coupled receptors
• Contains chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation