Methods in Enzymology Series, Vol. 416
Coordinator: Fukuda MinoruLanguage: Anglais
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456 p. · 15.2x22.9 cm · Hardback
Characterization of specific donor binding to £\1,4-N-acteylhexosaminyltransferase EXTL2 using Isothermal Titration Calorimetry.
NMR Analysis of Carbohydrate-Protein Interactions.
Determination of atomic structure for glycosyltransferase-substrate complex.
Section 2 ¡V Phage Display Library
Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates.
Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan epitopes.
Section 3 - Glycosyltransferase Expression Profiling
Autofluorescent proteins for monitoring the intracellular distribution of glycosyltransferases.
Expression profiling of glycosyltransferases and their related enzymes using in situ hybridization.
Expression profiling of glycosyltransferases using RT-PCR.
Expression profiling of lycosyltransferases and related enzymes using gene micro-arrays.
Section 4 - CHO mutants in Glycan Biosynthesis
Lectin Resistant CHO Glycosylation Mutants.
CHO glycosylation mutants ¡V GPI anchor.
CHO glycosylation mutants ¡Vproteoglycans.
Section 5 - Function of Sulfation
Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Protoglycans).
Measuring the activities of the sulfs, two novel heparin/heparan sulfate endosulfatases.
Determination of chemokine-glycosaminoglycan interaction specificity.
Identification of Proteoglycan-binding Proteins.
Functions of glycans revealed by gene inactivation of L-selectin ligand sulfotransferases in mice.
Section 6 - Functions Revealed by Forced Expression or Gene Knockout
Expression of specific carbohydrates by transfection with specific carbohydrate modifying enzymes.
N-glycan structure analysis using lectins and an a-mannosidase IIx activity assay.
Targeted disruption of the gene encoding core 1 ƒÒ1-3-galactosyltransferase (T-synthase) causes embryonic lethality and defective angiogenesis in mice.
Roles of Mucin-Type O-glycans Synthesized by Core2B1,6-N-aetylglucosaminyltransferase.
Analysis of leukocyte rolling in vivo and in vitro.
Cell type-dependent Roles of Carbohydrates in Tumor Metastasis.