Human Monoclonal Antibodies, 2014
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1060

The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.

1. Human Monoclonal Antibodies - The Residual Challenge of Antibody Immunogenicity

Herman Waldmann

2. Technical and Ethical Limitations in Making Human Monoclonal Antibodies

Mark C. Glassy and Rishab Gupta

3. Therapeutic Human Monoclonal Antibodies in Inflammatory Diseases

Sotirios Kotsovilis and Evangelos Andreakos

4. Therapeutic Human Monoclonal Antibodies Against Cancer

Jamie Jarboe, Anumeha Gupta, and Wasif Saif

5. Polyclonal and Monoclonal Antibodies in the Clinic

Bharath Wootla, Aleksandar Denic, and Moses Rodriguez

6. Production of Human Monoclonal Antibodies by the Epstein- Barr Virus Method

Michael Steinitz

7. Humanization and Simultaneous Optimization of Monoclonal Antibody

T. Kuramochi, T., Igawa, H. Tsunoda, and K. Hattori

8. Chimeric Antibodies

Kohei Kurosawa, Waka Lin, and Kunihiro Ohta

9. Recombinant Genetic Libraries and Human Monoclonal Antibodies

Jarrett J. Adams, Bryce Nelson, and Sachdev S. Sidhu

10. Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial Cytoplasm

Lilach Vaks and Itai Benhar

11. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgG-Single Chain Fv Fusion

Dan Lu and Zhenping Zhu

12. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display

André Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, and Michael Hust

13. Antigen –Specific Human Monoclonal Antibodies from Transgenic Mice

Susana Magadán Mompó and África González-Fernández

14. Phage Display Technology for Human Monoclonal Antibodies

Cecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, and Daniele Sblattero

15. Antigen-Specific in vitro Immunization: A Source for Human Monoclonal Antibodies

Kosuke Tomimatsu and Sanetaka Shirahata

16. Methods for Radiolabelling of Monoclonal Antibodies

Vladimir Tolmachev, Anna Orlova, and Karl Andersson

17. Purification of Human Monoclonal Antibodies and Their Fragments

Thomas Muller-Späth and Massimo Morbidelli

18. Idiotype-Specific IVIG for Therapy of Autoimmune Diseases

Tomer Bashi, Miri Blank, and Yehuda Shoenfeld

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts