Description
Laboratory Methods in Enzymology: Protein Part C
Author: LORSCH Jon
Language: EnglishSubjects for Laboratory Methods in Enzymology: Protein Part C:
296 p. · 15x22.8 cm · Hardback
Description
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In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.
TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein–Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Indispensable tool for the researcher
- Carefully written and edited by experts to contain step-by-step protocols
- In this volume we have brought together a number of core protocols concentrating on Protein