Laser Manipulation of Cells and Tissues
Methods in Cell Biology Series, Vol. 82
Coordinators: Berns Michael W., Greulich Karl OttoLanguage: Anglais
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640 p. · 19.1x23.5 cm · Hardback
* Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research
* Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology
* Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers
Selected Applications of Laser Scissors and Tweezers and New Applications in Heart Research.
Mechanisms of Laser Cellular Microsurgery.
Cellular and Colloidal Separation using Optical Forces.
Laser Microsurgery in the GFP Era – A Cell Biologist’s Perspective.
Investigating Relaxation Processes in Cells and Developing Organisms: From Cell Ablation to Cytoskeleton Nanosurgery.
Fs-Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells and Studies of Cell Mechanics.
Chromophore-Assisted Laser Inactivation (CALI).
Laser Surgery and Optical Trapping in a Laser Scanning Microscope.
Investigation of Laser Microdissected-Inclusion Bodies.
In situ Analysis of DNA Damage Response and Repair Using Laser Microirradiation.
Laser Effects in the Manipulation of Human Eggs and Embryos for In Vitro Fertilization.
Laser Microdissection-Based Analysis of Plant Sex Chromosomes.
Rapid Sampling for Single-Cell Analysis by Capillary Electrophoresis.
Physics of Optical Tweezers.
Laser Tweezers in the Study of Mechanobiology in Live Cells.
Optical Micromanipulation Methods for Controlled Rotation, Transportation and Microinjection of Biological Objects.
Optical Torque on Microscopic Objects.
Automated Motile Cell Capture and Analysis with Optical Traps.
Principles of Laser Microdissection and Catapulting of Histologic Specimens and Live Cells.
Non-contact Laser Microdissection and Catapulting for Pure Sample Capture.
Laser Capture Microdissection and Laser Pressure Catapulting as Tools to Study Gene Expression in Individual Cells of a Complex Tissue.
Laser Capture Microdissection in Comparative Proteomics Analysis of Hepatocellular Carcinoma.
Laser-based Measurements in Cell Biology.
Appendix: Practical Aspects of Working with Laser Microbeams and Optical Tweezers.