LC-MS in Drug Analysis, Softcover reprint of the original 1st ed. 2012
Methods and Protocols
Methods in Molecular Biology Series, Vol. 902

Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance.  The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques.  The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents.  Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

1. An Introduction to Drug Testing – The Expanding Role of Mass Spectrometry

            Catherine Hammett-Stabler and Steven W. Cotton

2. LC-MS vs. GC-MS, Online Extraction Systems, Advantages of Technology for Drug Screening Assays

            Pierre Marquet

3. LC-MS/MS Techniques for High Volume Screening of Drugs of Abuse and Target Drug Quantitation in Urine/Blood Matrices

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras