Measuring Biological Responses with Automated Microscopy
Methods in Enzymology Series, Vol. 414

Editor-in-Chief: Inglese James

Language: Anglais
Cover of the book Measuring Biological Responses with Automated Microscopy

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
Dynamic GFP Sensors for High-Content Analysis of the Cell Cycle
High Content Fluorescence-based Screening for Epigenetic Modulators
Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins
The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan GPCRs by Arrestin Recruitment
High Content Screening of Known G Protein-Coupled Receptors by Arrestin Translocation
Cell Imaging Assays for G protein-coupled Receptor Internalization: Application to High Throughput Screening
High throughput confocal microscopy for beta arrestin-GFP translocation GPCR assays using the Evotec Opera
G-protein coupled receptor internalization assays in the High Content Screening Format
Screening for Activators of the Wnt/Fzd Pathway by Automated Fluorescent Microscopy
A live-cell, image based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation
Quantitative single cell studies of nuclear receptor function using high-resolution HTM and image analyses
Tracking the motion of individual proteins with semiconductor quantum dots
Development and Application of Automatic High Resolution Light Microscopy for Cell Based Screens
Adenoviral Sensors for High-Content Cellular Analysis
Cell based assays in primary endothelial cells to study multiple steps in inflammation
Development and Implementation of Multiplexed Cell-Based Imaging Assays
High throughput screening for modulators of stem cell differentiation
High Content Kinetic Calcium Imaging in Drug Sensitive and Drug Resistant Human Breast Cancer Cells
Measurement and Analysis of Calcium Signaling in Heterogeneous Cell Cultures
Mulipliex Analysis of Inflammatory Signalling Pathways using a High Content Imaging System
Generation and Characterization of a Stable MK2-EGFP Cell Line and Subswquent Development of a High Content Imaging Assay on the Cellomics ArrayScan® Platform to Screen for p38 MAPK Inhibitors
Development and implementation of three MAPK signaling pathway imaging assays to provide MAPK module selectivity profiling for kinase inhibitors; MK2-EGFP translocation, c-Jun & ERK activation.
Assay Development and Case History of a 32K Biased Library High Content MK2-EGFP Translocation Screen to Identify p38 MAPK Inhibitors on the ArrayScan® 3.1 Imaging Platform
Development and Implementation of a dual target MAPK high content assay (MK2-EGFP translocation & cJun activation) to provide LO support for kinase inhibitors
Compound classification using image-based cellular phenotypes
High Content Screening: Emerging Hardware and Software Technologies
An Infrastructure for High Throughput Microscopy: Instrumentation, Informatics, and Integration
Protein Translocation Assays: Key Tools for Accessing New Biological Information with High Throughput Microscopy
High-Content Screening of Functional Genomic Libraries
Fluorescent protein-based cellular assays analyzed by laser scanning microplate cytometry in 1536-well plate format
High-Throughput Measurements of Biochemical Responses Using the Plate::Vision™ Multimode 96 Mini-lens Array Reader
Systems Cell Biology Based on High Content Screening
Digital Autofocus Methods for Automated Microscopy
Fluorescence Lifetime Imagine Microscopy – Two-dimensional distribution measurement of fluorescence lifetime
Biochmemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists