Description
Metalloproteins, 2014
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1122
Coordinators: Fontecilla-Camps Juan C., Nicolet Yvain
Language: EnglishSubject for Metalloproteins:
Publication date: 09-2016
Support: Print on demand
Publication date: 03-2014
299 p. · 17.8x25.4 cm · Hardback
Description
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Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.
1. Advances in Research on Metalloproteins
Perry A. Frey
2. Purification of O2-Sensitive Metalloproteins
Carlos Echavarri-Erasun, Simon Arragain, and Luis M. Rubio
3. Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources
Carlos Echavarri-Erasun, Simon Arragain, Alessandro A. Scandurra, and Luis M. Rubio
4. Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins
Jason C. Crack, Jeffrey Green, Andrew J. Thomson, and Nick E. Le Brun
5. Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers
Jon M. Kuchenreuther, Stacey A. Shiigi, and James R. Swartz
6. Electrochemistry of Metalloproteins – Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1
Rhiannon M. Evans and Fraser A. Armstrong
7. FTIR Spectroscopy of Metalloproteins
Oscar Gutiérrez-Sanz, Olaf Rüdger, and Antonio L. De Lacey
8. Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy
Hirotoshi Matsumura and Pierre Moënne-Loccoz
9. A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds
Hongxin Wang, Ercan Alp, Yoshitaka Yoda, and Stephen P. Cramer
10. Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR)
Serge Gambarelli and Vincent Maurel
11. Mössbauer Spectroscopy
Martin Clémancey, Geneviève Blondin, Jean-Marc Latour, and Ricardo Garcia-Serres
12. X-Ray Absorption Spectroscopy of Metalloproteins
Jesse Ward, Emily Ollmann, Evan Maxey, and Lydia A. Finney
13. X-Ray Crystallographic Studies of Metalloproteins
Anne Volbeda
14. Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds
Luca Bertini, Maurizion Bruschi, Ugo Cosentino, Claudio Greco, Giorgio Moro, Giuseppe Zampella, and Luca De Gioia
15. Density Functional Theory – Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups
Jean-Marie Mouesca
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
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