Mitochondrial Function

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function.

1. High-Content Functional Genomic Screening to Identify Novel Regulators of the PINK1-Parkin Pathway
Andy Cheuk-Him Ng, Stephen Baird and Robert A. Screaton
2. Measurement of Mitochondrial Turnover and Life Cycle using MitoTimer
Kyle M. Trudeau , Roberta A.Gottlieb and Orian S. Shirihai
3. Monitoring Mitophagy in Mammalian Cells
Yushan Zhu , Guo Chen, Linbo Chen, Du Feng, Lei Liu and Quan Chen
4. Photoactivatable Green Fluorescent Protein-Based Visualization and Quantification of Mitochondrial Fusion and Mitochondrial Network Complexity in Living Cells
Mariusz Karbowski, Megan M. Cleland and Brian Roelofs
5. Characterization of Mitochondrial Transport in Neurons
Bing Zhou, Mei-Yao Lin, Tao Sun, Adam L. Knight and Zu-Hang Sheng
6. Imaging of Mitochondrial Dynamics in Motor and Sensory Axons of Living Mice.
Irene Bolea, Wen-Biao Gan, Giovanni Manfedi and Jordi Magranél
7. Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures
Anh H. Pham and David C. Chan
8. Analysis of Mitochondrial Traffic in Drosophila
Swathi Devireddy, Hyun Sung, Pin-Chao Liao, Elisabeth Garland-Kuntz and Peter J Hollenbeck
9. In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae
Dominik Paquet , Gabriela Plucinska and Thomas Misgeld
10. The Use of MiniSOG in the Localization of Mitochondrial Proteins
Guy A. Perkins
11. Assessing the Function of Mitochondria-Associated ER Membranes
Estela Area-Gomez.
12. Measurement of ROS Homeostasis in Isolated Mitochondria
L. Tretter, and A. Ambrus
13. Use of Potentiometric Fluorophores in the Measurement of Mitochondrial Reactive Oxygen Species
Brian M. Polster , David G. Nicholls , Shealinna X. Ge and Brian A. Roelofs
14. Spatial, Temporal and Quantitative Manipulation of Intracellular Hydrogen Peroxide in Cultured Cells
Ishraq Alim, Renee E Haskew-Layton, Hossein Aleyasin, Hengchang Guo and Rajiv R Ratan
15. Biochemical and Biophysical Methods for Studying Mitochondrial Iron Metabolism
Gregory P. Holmes-Hampton, Wing-Hang Tong and Tracey A. Rouault
16. Analysis and Interpretation of Microplate-Based Oxygen Consumption and PH Data
Ajit S. Divakaruni, Alexander Paradyse1, David A. Ferrick, Anne N. Murphy ,and Martin Jastroch
17. Imaging Changes in the Cytosolic ATP-to-ADP ratio
Mathew Tantama and  Gary Yellen
18. The Use of MiniSOG in the Localization of Mitochondrial Proteins
Carlos T. Moraes
19. Induced Pluripotent Stem Cell Derived Models for MtDNA Diseases
Riikka H Hämäläinen
20. The Use of 18F-BCPP-EF as a PET Probe for Complex I Activity in the Brain
Hideo Tsukada
21. MR OEF Imaging in MELAS
Sheng Xie